ArticleJournal of bacteriology2008
A new TetR family transcriptional regulator required for morphogenesis in Streptomyces coelicolor.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Physiological Functions of the Cello-Oligosaccharides Binding CebE in the PathogenicMicroorganisms · 2024Article
- The Global Regulator MftR Controls Virulence and Siderophore Production in Burkholderia thailandensis.Journal of bacteriology · 2022Article
- Extracytoplasmic Function Sigma Factors Governing Production of the Primary Siderophores in PathogenicFrontiers in microbiology · 2022Review
- Coelimycin Synthesis Activatory Proteins Are Key Regulators of Specialized Metabolism and Precursor Flux inFrontiers in microbiology · 2021Article
- A genome-wide approach for identification and characterisation of metabolite-inducible systems.Nature communications · 2020Article
- The Link between Purine Metabolism and Production of Antibiotics inAntibiotics (Basel, Switzerland) · 2019Review
- Dissecting and engineering of the TetR family regulator SACE_7301 for enhanced erythromycin production in Saccharopolyspora erythraea.Microbial cell factories · 2014Article
- SACE_3986, a TetR family transcriptional regulator, negatively controls erythromycin biosynthesis in Saccharopolyspora erythraea.Journal of industrial microbiology & biotechnology · 2014Article
- Regulatory role of tetR gene in a novel gene cluster of Acidovorax avenae subsp. avenae RS-1 under oxidative stress.Frontiers in microbiology · 2014Article
- The TetR family of regulators.Microbiology and molecular biology reviews : MMBR · 2013Review
- Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter.Biotechnology for biofuels · 2013Article
- LplR, a repressor belonging to the TetR family, regulates expression of the L-pantoyl lactone dehydrogenase gene in Rhodococcus erythropolis.Applied and environmental microbiology · 2012Article
- Genome context as a predictive tool for identifying regulatory targets of the TetR family transcriptional regulators.PloS one · 2012Article
- Regulation of transcription by SMU.1349, a TetR family regulator, in Streptococcus mutans.Journal of bacteriology · 2011Article
- Identification of SACE_7040, a member of TetR family related to the morphological differentiation of Saccharopolyspora erythraea.Current microbiology · 2011Article
- SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes.BMC microbiology · 2011Article
- CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus.Journal of bacteriology · 2009Article
- Cif is negatively regulated by the TetR family repressor CifR.Infection and immunity · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Both morphogenesis and antibiotic production in the streptomycetes are initiated in response to starvation, and these events are coupled. We previously described a transposon-generated mutant in Streptomyces coelicolor, SE293, that resulted in a bld strain that overproduced the antibiotic actinorhodin. The SCO1135 open reading frame identified by the insertion encodes a member of the TetR family of transcriptional regulators. Here we show that a constructed deletion of the SCO1135 open reading frame resulted in the same morphological and antibiotic production phenotype as the insertion mutant. The constructed deletion also resulted in constitutive expression of SCO1135 transcript, as well as that of the gene cluster immediately adjacent to it, SCO1134-1132, which encodes a putative molybdopterin binding complex. A His6-tagged version of the SCO1135 protein product was shown to bind the intergenic region between SCO1135 and SCO1134, which contains the apparent transcription start sites for each gene mapped by primer extension analysis. Increased expression of the SCO1134-1132 transcript in the SCO1135 deletion mutant also resulted in increased expression of xanthine dehydrogenase activity, confirming the predictions about these open reading framed based on protein similarity. We have designated the SCO1134-1142 gene cluster xdhABC and the regulator encoded by SCO1135 xdhR. We speculate that the inappropriate expression of xanthine dehydrogenase affects purine salvaging pathways at the onset of development, creating artificially high concentrations of both GTP and ppGpp and perturbing the pathways these molecules participate in for the initiation of morphogenesis and antibiotic production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.