ArticleJournal of bacteriology2008
Phosphoketolase pathway dominates in Lactobacillus reuteri ATCC 55730 containing dual pathways for glycolysis.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 93 citations in OpenAlex.
- Single-Step Production of Mannitol From Yacon Fructo-Oligosaccharides Using Fructophilic Lactic Acid Bacteria.MicrobiologyOpen · 2026Article
- Pseudomonas aeruginosa Relies on a Phosphoketolase to Support Anaerobic Survival Under Reductive Stress.Molecular microbiology · 2026Article
- Ulvan Polysaccharide ofInternational journal of food science · 2026Article
- In silico interactome analysis reveals distinct and complementary metabolic roles of bacteria in stingless bee larval food.Computational and structural biotechnology journal · 2026Article
- Lactic Acid Production from Sugarcane Bagasse Hydrolysates byACS omega · 2025Article
- Phosphoketolase and KDPG aldolase metabolisms modulate photosynthetic carbon yield in cyanobacteria.The Plant cell · 2024Article
- Development and implementation of a simulated microgravity setup for edible cyanobacteria.NPJ microgravity · 2024Article
- Article
- Oxygen Transfer Effect on the Growth of Limosilactobacillus reuteri ATCC 53608 and on Its Metabolic Capacity.Current microbiology · 2024Article
- Microbial ensemble in the hives: deciphering the intricate gut ecosystem of hive and forager bees of Apis mellifera.Molecular biology reports · 2024Article
- Non-inhibitory levels of oxygen during cultivation increase freeze-drying stress tolerance inFrontiers in microbiology · 2023Article
- Journey of the Probiotic Bacteria: Survival of the Fittest.Microorganisms · 2022Review
- Mannitol Production by Heterofermentative Lactic Acid Bacteria: a Review.Applied biochemistry and biotechnology · 2022Review
- Article
- Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri.BMC biotechnology · 2021Article
- A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory.Microbial cell factories · 2019Article
- High Relative Abundance ofNutrients · 2019Article
- Pareto Optimality Explanation of the Glycolytic Alternatives in Nature.Scientific reports · 2019Article
- Whole-genome-based phylogeny of Bacillus cytotoxicus reveals different clades within the species and provides clues on ecology and evolution.Scientific reports · 2019Article
- Coconut milk beverage fermented byJournal of food science and technology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic flux analysis indicated that the heterofermentative Lactobacillus reuteri strain ATCC 55730 uses both the Embden-Meyerhof pathway (EMP) and phosphoketolase pathway (PKP) when glucose or sucrose is converted into the three-carbon intermediate stage of glycolysis. In all cases studied, the main flux is through the PKP, while the EMP is used as a shunt. In the exponential growth phase, 70%, 73%, and 84% of the flux goes through the PKP in cells metabolizing (i) glucose plus fructose, (ii) glucose alone, and (iii) sucrose alone, respectively. Analysis of the genome of L. reuteri ATCC 55730 confirmed the presence of the genes for both pathways. Further evidence for the simultaneous operation of two central carbon metabolic pathways was found through the detection of fructose-1,6-bisphosphate aldolase, phosphofructokinase, and phosphoglucoisomerase activities and the presence of phosphorylated EMP and PKP intermediates using in vitro 31P NMR. The maximum specific growth rate and biomass yield obtained on glucose were twice as low as on sucrose. This was the result of low ATP levels being present in glucose-metabolizing cells, although the ATP production flux was as high as in sucrose-metabolizing cells due to a twofold increase of enzyme activities in both glycolytic pathways. Growth performance on glucose could be improved by adding fructose as an external electron acceptor, suggesting that the observed behavior is due to a redox imbalance causing energy starvation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.