Evidence map›Paper›PMID 17964628›Full record

ArticleVirology2008

TY3 GAG3 protein forms ordered particles in Escherichia coli.

Liza S Z Larsen, Yurii Kuznetsov, Alex McPherson, G Wesley Hatfield, Suzanne Sandmeyer

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

  1. Structure of the Ty3/Gypsy retrotransposon capsid and the evolution of retroviruses.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  2. Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Liza S Z LarsenDepartment of Biological Chemistry, University of California, Irvine, CA 92697, USA.
Yurii Kuznetsov
Alex McPherson
G Wesley Hatfield
Suzanne Sandmeyer
University of California, Irvine · US

Funding

Ty3 Viruslike Particle Morphogenesis and Host InteractionsR01GM033281 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 1985 to 2013
$4.7M
STRUCTURE OF VIRAL RNA AND ITS ROLE IN ASSEMBLYR01GM058868 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI MCPHERSON, ALEXANDER · 1999 to 2006
$2.3M
Global Regulatory Networks in Escherichia ColiR01GM068903 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI HATFIELD, G. WESLEY · 2003 to 2006
$1.3M
NIGMS NIH HHS GM33281NIGMS NIH HHS GM58868NIGMS NIH HHS GM68903NIGMS NIH HHS R01 GM033281NIGMS NIH HHS R01 GM058868NIGMS NIH HHS R01 GM068903
6 · The paper itself

Abstract

The yeast retrovirus-like element Ty3 GAG3 gene encodes a Gag3 polyprotein analogous to retroviral Gag. Gag3 lacks matrix, but contains capsid, spacer, and nucleocapsid domains. Expression of a Ty3 Gag3 or capsid domain optimized for expression in Escherichia coli was sufficient for Ty3 particle assembly. Virus-like ordered particles assembled from Gag3 were similar in size to immature particles from yeast and contained nucleic acid. However, particles assembled from the CA domain were variable in size and displayed much less organization than native particles. These results indicate that assembly can be driven through interactions among capsid subunits in the particle, but that the nucleocapsid domain, likely in association with RNA, confers order upon this process.

Indexed as

Adaptor Proteins, Signal TransducingBase SequenceCapsid ProteinsCarrier ProteinsCloning, MolecularDNA PrimersEscherichia coliGene ExpressionGenes, FungalMicroscopy, Atomic ForceRecombinant ProteinsRetroelementsRNA-Directed DNA PolymeraseSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTransformation, GeneticAdaptor Proteins, Signal TransducingCapsid ProteinsCarrier ProteinsDNA PrimersMDV1 protein, S cerevisiaeRecombinant ProteinsRetroelementsreverse transcriptase Ty3, S cerevisiaeRNA-Directed DNA PolymeraseSaccharomyces cerevisiae Proteins

Identifiers

PMID17964628
PMCPMC2701475
OpenAlexW2016610930

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.