ArticleFungal genetics and biology : FG & B2008
A single SNP, G929T (Gly310Val), determines the presence of a functional and a non-functional allele of HIS4 in Candida albicans SC5314: detection of the non-functional allele in laboratory strains.
Article in Fungal genetics and biology : FG & B, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Targeted loss of heterozygosity in Candida albicans using CRISPR-Cas9 reveals the functional impact of allelic variation.Genetics · 2025Article
- Single-cell detection of copy number changes reveals dynamic mechanisms of adaptation to antifungals in Candida albicans.Nature microbiology · 2024Article
- Probing gene function inmSphere · 2024Article
- The Impact of Gene Dosage and Heterozygosity on The Diploid PathobiontJournal of fungi (Basel, Switzerland) · 2019Review
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- Article
- Phenotypic Consequences of a Spontaneous Loss of Heterozygosity in a Common Laboratory Strain of Candida albicans.Genetics · 2016Article
- Post-transcriptional regulation of transcript abundance by a conserved member of the tristetraprolin family in Candida albicans.Molecular microbiology · 2015Article
- A FACS-optimized screen identifies regulators of genome stability in Candida albicans.Eukaryotic cell · 2015Article
- One-step targeted gene deletion in Candida albicans haploids.Nature protocols · 2014Article
- An MDR1 promoter allele with higher promoter activity is common in clinically isolated strains of Candida albicans.Molecular genetics and genomics : MGG · 2011Article
- Rad52 function prevents chromosome loss and truncation in Candida albicans.Molecular microbiology · 2011Article
- Loss of heterozygosity at an unlinked genomic locus is responsible for the phenotype of a Candida albicans sap4Δ sap5Δ sap6Δ mutant.Eukaryotic cell · 2011Article
- Role of the homologous recombination genes RAD51 and RAD59 in the resistance of Candida albicans to UV light, radiomimetic and anti-tumor compounds and oxidizing agents.Fungal genetics and biology : FG & B · 2010Article
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5 authors.
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Abstract
Candida albicans is a diploid organism that exhibits high levels of heterozygosity. Although the precise manner by which this heterozygosity provides advantage for the commensal/pathogenic life styles of C. albicans is not known, heterozygous markers are themselves useful for studying genomic rearrangements, which occur frequently in C. albicans. Treatment of CAI-4 with UV light yielded histidine auxotrophs which could be complemented by HIS4, suggesting that strain CAI-4 is heterozygous for HIS4. These auxotrophs appeared to have undergone mitotic recombination and/or chromosome loss. As expected from a heterozygote, disruption of the functional allele of HIS4 resulted in a his4::hisG-URA3-hisG strain that is auxotrophic for histidine. Sequencing of random clones of the HIS4 ORF from CAI-4 and its precursor SC5314 revealed the presence of 11 SNPs, seven synonymous and four non-synonymous. Site-directed mutagenesis indicates that only one of those SNPs, T929G (Gly310Val), is responsible for the non-functionality of the encoded enzyme. HIS4 analysis of five commonly used laboratory strains is reported. This study provides a new, easily measured nutritional marker that can be used in future genetic studies in C. albicans.
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