ArticleInvestigative ophthalmology & visual science2007
Neutrophil interactions with keratocytes during corneal epithelial wound healing: a role for CD18 integrins.
Article in Investigative ophthalmology & visual science, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Impact of Obesity and Age on Mouse Corneal Innervation at the Epithelial-Stromal Interface.Investigative ophthalmology & visual science · 2024Article
- Ly6GResearch (Washington, D.C.) · 2024Article
- Posterior corneoscleral limbus: Architecture, stem cells, and clinical implications.Progress in retinal and eye research · 2023Review
- An Epithelial Abrasion Model for Studying Corneal Wound Healing.Journal of visualized experiments : JoVE · 2021Article
- Review
- Visualizing the Contribution of Keratin-14Stem cell reports · 2019Article
- Serial block-face scanning electron microscopy reveals neuronal-epithelial cell fusion in the mouse cornea.PloS one · 2019Article
- Hyperopic refractive correction by LASIK, SMILE or lenticule reimplantation in a non-human primate model.PloS one · 2018Article
- IL-20 promotes epithelial healing of the injured mouse cornea.Experimental eye research · 2017Article
- Inhibition of Ultraviolet B-Induced Expression of the Proinflammatory Cytokines TNF-α and VEGF in the Cornea by Fucoxanthin Treatment in a Rat Model.Marine drugs · 2016Article
- Platelet recruitment promotes keratocyte repopulation following corneal epithelial abrasion in the mouse.PloS one · 2015Article
- The integrin needle in the stromal haystack: emerging role in corneal physiology and pathology.Journal of cell communication and signaling · 2014Article
- Effects of vitamin D receptor knockout on cornea epithelium gap junctions.Investigative ophthalmology & visual science · 2014Article
- Cytokine deposition alters leukocyte morphology and initial recruitment of monocytes and γδT cells after corneal injury.Investigative ophthalmology & visual science · 2014Article
- Wounding the cornea to learn how it heals.Experimental eye research · 2014Review
- Integrin-dependent neutrophil migration in the injured mouse cornea.Experimental eye research · 2014Article
- Article
- Using bovine pituitary extract to increase proliferation of keratocytes and maintain their phenotype in vitro.International journal of ophthalmology · 2013Article
- Article
- Dendritic cell-epithelium interplay is a determinant factor for corneal epithelial wound repair.The American journal of pathology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
purposeTo determine the role of keratocytes and leukocyte beta(2) (CD18) integrins in neutrophil (PMN) migration through the corneal stroma after epithelial scrape injury.
methodsUsing C57BL/6 wild-type and CD18(-/-) mice, corneas were excised at 6 hours (wild-type) or 24 hours (CD18(-/-)) after central corneal epithelial abrasion, time points determined previously to have similar levels of emigrated PMNs. Corneas were prepared for ultrastructural morphometric analysis of PMNs, keratocyte networks, and collagen.
resultsTransmission electron microscopy revealed intact keratocyte networks within the paralimbus that were morphometrically similar, regardless of epithelial injury or mouse genotype. Secondary to epithelial abrasion, extravasated PMNs within the paralimbus developed close contacts with keratocytes and collagen. In wild-type mice, 40% of the PMN surface was in contact with the keratocyte surface, and this value decreased to 10% in CD18(-/-) mice. PMN contact with collagen was similar in wild-type and CD18(-/-) mice, with approximately 50% of the PMN surface contacting the collagen fibrils. Since corneal edema resulting from scrape injury was similar, regardless of genotype and did not involve structural changes in collagen fibrils, these data favor a direct role for CD18 in mediating PMN contact with keratocytes.
conclusionsThe data show that in response to epithelial scrape injury, PMN migration in the corneal stroma involves close contact between keratocytes and collagen. Although PMN-keratocyte contacts require CD18 integrins, contact with collagen is CD18 independent. Fundamentally, PMN migration along keratocyte networks constitutes the beginning of a new experimental concept for understanding leukocyte migration within the wounded cornea.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.