ArticleMolecular endocrinology (Baltimore, Md.)2008
Transcriptome profiling of estrogen-regulated genes in human primary osteoblasts reveals an osteoblast-specific regulation of the insulin-like growth factor binding protein 4 gene.
Article in Molecular endocrinology (Baltimore, Md.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
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- Identification of thyroid hormone response genes in the remodeling of dorsal muscle duringFrontiers in endocrinology · 2023Article
- Role of IGF1 and EFN-EPH signaling in skeletal metabolism.Journal of molecular endocrinology · 2018Review
- Estrogens and Androgens in Skeletal Physiology and Pathophysiology.Physiological reviews · 2017Review
- Skeletal effects of growth hormone and insulin-like growth factor-I therapy.Molecular and cellular endocrinology · 2016Review
- Article
- Sex steroid actions in male bone.Endocrine reviews · 2014Review
- The many faces of estrogen signaling.Biochemia medica · 2014Review
- Metastasin S100A4 is a mediator of sex hormone-dependent formation of the cortical bone.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Direct transcriptional targets of sex steroid hormones in bone.Journal of cellular biochemistry · 2011Review
- Quantitative nanoproteomics for protein complexes (QNanoPX) related to estrogen transcriptional action.Molecular & cellular proteomics : MCP · 2010Article
- Microarray analysis of altered gene expression in ERbeta-overexpressing HEK293 cells.Endocrine · 2009Article
- Estrogen receptor alpha represses transcription of early target genes via p300 and CtBP1.Molecular and cellular biology · 2009Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estradiol (E2) is believed to modulate physiological functions relevant to osteoblast biology through the actions of estrogen receptors (ERs) that in turn regulate the expression of target genes. The molecular effects of estrogen action in bone remain to be fully elucidated. This study reports a genome-wide molecular and computational analysis of the interaction between ER and regulatory elements on the DNA of target genes in human primary osteoblasts. Of approximately 54,000 gene probes surveyed in this study, a total of 375 genes were up-regulated and 418 genes were down-regulated on exposure to E2, with only 46 of these being direct target genes after 24 h, as determined by concomitant cycloheximide treatment. Computational analysis discovered several pathways where E2 co-regulates multiple functionally linked components. Examination of the genomic sequence of IGF binding protein 4 located ER response elements within the first intron. Using by chromatin immunoprecipitation, we show a site- and cell-specific recruitment of transcription factors to this newly identified regulatory region. Transient transfection studies revealed that this intronic region acts as a functional promoter in human osteoblasts. Taken together, this analysis provides a comprehensive gene transcription profile and identifies several genes of potential physiological importance in controlling estrogen-mediated signaling in primary osteoblasts.
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