ArticleJournal of structural biology2008
The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications.
Article in Journal of structural biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed.
- Structural and Functional Characterization of EXPO-Derived Extracellular Vesicles in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Ultrastructure of astrocytes using volume electron microscopy: A scoping review.The Journal of physiology · 2026Article
- Seminal plasma exosomes improve the motility and mitochondrial function of goat spermatozoa during liquid storage by regulating oxidative phosphorylation.Stress biology · 2025Article
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- ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ.Nature protocols · 2025Review
- Transformative insights in breast cancer: review of atomic force microscopy applications.Discover oncology · 2025Review
- Evaluating ultrastructural preservation quality in banked brain tissue.Free neuropathology · 2025Article
- Visualization of Bacillus subtilis spore structure and germination using quick-freeze deep-etch electron microscopy.Microscopy (Oxford, England) · 2024Article
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- Cryopreservation of brain cell structure: a review.Free neuropathology · 2024Article
- Imaging Bunyavirus Infections by Transmission Electron Microscopy: Conventional Sample Preparation vs High-Pressure Freezing and Freeze-Substitution.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Rehydration of Freeze Substituted Brain Tissue for Pre-embedding Immunoelectron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
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- Structural Diversity within the Endoplasmic Reticulum-From the Microscale to the Nanoscale.Cold Spring Harbor perspectives in biology · 2023Review
- A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility.Cell · 2022Article
- Morphological principles of neuronal mitochondria.The Journal of comparative neurology · 2022Article
- Dissection and ultramicroscopic observation of an apical pollen tube of Pyrus.Plant reproduction · 2022Article
- Three-dimensional virtual histology of the cerebral cortex based on phase-contrast X-ray tomography.Biomedical optics express · 2021Article
- Article
- Nano-scale morphology of cardiomyocyte t-tubule/sarcoplasmic reticulum junctions revealed by ultra-rapid high-pressure freezing and electron tomography.Journal of molecular and cellular cardiology · 2021Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its own challenges for the preparation of thick sections. High pressure freezing in combination with freeze substitution provides the best method for obtaining the largest volume of well-preserved tissue. However, for deeply embedded, heterogeneous, labile tissues needing careful dissection, such as brain, the damage due to anoxia and excision before cryofixation is significant. We previously demonstrated that chemical fixation prior to high pressure freezing preserves fragile tissues and produces superior tomographic reconstructions compared to equivalent tissue preserved by chemical fixation alone. Here, we provide further characterization of the technique, comparing the ultrastructure of Flock House Virus infected DL1 insect cells that were (1) high pressure frozen without fixation, (2) high pressure frozen following fixation, and (3) conventionally prepared with aldehyde fixatives. Aldehyde fixation prior to freezing produces ultrastructural preservation superior to that obtained through chemical fixation alone that is close to that obtained when cells are fast frozen without fixation. We demonstrate using a variety of nervous system tissues, including neurons that were injected with a fluorescent dye and then photooxidized, that this technique provides excellent preservation compared to chemical fixation alone and can be extended to selectively stained material where cryofixation is impractical.
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