ArticleMolecular biology of the cell2008
Distinct pathways for the early recruitment of myosin II and actin to the cytokinetic furrow.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Regionalized regulation of actomyosin organization influences cardiomyocyte cell shape changes during chamber curvature formation.Nature communications · 2026Article
- Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.Frontiers in cell and developmental biology · 2024Article
- Rapid assembly of a polar network architecture drives efficient actomyosin contractility.Cell reports · 2022Article
- Filament-guided filament assembly provides structural memory of filament alignment during cytokinesis.Developmental cell · 2021Article
- Ventral stress fibers induce plasma membrane deformation in human fibroblasts.Molecular biology of the cell · 2021Article
- Connecting cell polarity signals to the cytokinetic machinery in yeast and metazoan cells.Cell cycle (Georgetown, Tex.) · 2021Review
- Article
- Polar relaxation by dynein-mediated removal of cortical myosin II.The Journal of cell biology · 2020Article
- Chromokinesin KIF4A teams up with stathmin 1 to regulate abscission in a SUMO-dependent manner.Journal of cell science · 2020Article
- LIS1 determines cleavage plane positioning by regulating actomyosin-mediated cell membrane contractility.eLife · 2020Article
- Comparative Analysis of the Roles of Non-muscle Myosin-IIs in Cytokinesis in Budding Yeast, Fission Yeast, and Mammalian Cells.Frontiers in cell and developmental biology · 2020Review
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- Molecular form and function of the cytokinetic ring.Journal of cell science · 2019Review
- Network Contractility During Cytokinesis-from Molecular to Global Views.Biomolecules · 2019Review
- Molecular mechanisms of contractile-ring constriction and membrane trafficking in cytokinesis.Biophysical reviews · 2018Review
- A positive-feedback-based mechanism for constriction rate acceleration during cytokinesis ineLife · 2018Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
- Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator.Nature communications · 2017Article
- Analysis of interphase node proteins in fission yeast by quantitative and superresolution fluorescence microscopy.Molecular biology of the cell · 2017Article
- Article
4 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
Equatorial organization of myosin II and actin has been recognized as a universal event in cytokinesis of animal cells. Current models for the formation of equatorial cortex favor either directional cortical transport toward the equator or localized de novo assembly. However, this process has never been analyzed directly in dividing mammalian cells at a high resolution. Here we applied total internal reflection fluorescence microscope (TIRF-M), coupled with spatial temporal image correlation spectroscopy (STICS) and a new analytical approach termed temporal differential microscopy (TDM), to image the dynamics of myosin II and actin during the assembly of equatorial cortex. Our results indicated distinct and at least partially independent mechanisms for the early equatorial recruitment of myosin and actin filaments. Cortical myosin showed no detectable directional flow during early cytokinesis. In addition to equatorial assembly, we showed that localized inhibition of disassembly contributed to the formation of the equatorial myosin band. In contrast to myosin, actin filaments underwent a striking flux toward the equator. Myosin motor activity was required for the actin flux, but not for actin concentration in the furrow, suggesting that there was a flux-independent, de novo mechanism for actin recruitment along the equator. Our results indicate that cytokinesis involves signals that regulate both assembly and disassembly activities and argue against mechanisms that are coupled to global cortical movements.
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