ArticleNucleic acids research2007
Interaction of SmpB with ribosome from directed hydroxyl radical probing.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Bacterial Ribosome Rescue Systems.Microorganisms · 2022Review
- Review
- Ribosome-based quality control of mRNA and nascent peptides.Wiley interdisciplinary reviews. RNA · 2017Review
- ArfA recognizes the lack of mRNA in the mRNA channel after RF2 binding for ribosome rescue.Nucleic acids research · 2014Article
- Rejection of tmRNA·SmpB after GTP hydrolysis by EF-Tu on ribosomes stalled on intact mRNA.RNA (New York, N.Y.) · 2014Article
- The bacterial translation stress response.FEMS microbiology reviews · 2014Review
- Resolving nonstop translation complexes is a matter of life or death.Journal of bacteriology · 2014Review
- Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ.Nucleic acids research · 2014Article
- An unusual mechanism for EF-Tu activation during tmRNA-mediated ribosome rescue.RNA (New York, N.Y.) · 2014Article
- Trans-translation exposed: understanding the structures and functions of tmRNA-SmpB.Frontiers in microbiology · 2014Review
- The SmpB C-terminal tail helps tmRNA to recognize and enter stalled ribosomes.Frontiers in microbiology · 2014Review
- Mechanism of trans-translation revealed by in vitro studies.Frontiers in microbiology · 2014Review
- Distinct tmRNA sequence elements facilitate RNase R engagement on rescued ribosomes for selective nonstop mRNA decay.Nucleic acids research · 2014Article
- tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell.Frontiers in genetics · 2014Review
- Decoding in the absence of a codon by tmRNA and SmpB in the ribosome.Science (New York, N.Y.) · 2012Article
- Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.Science (New York, N.Y.) · 2012Article
- Bifunctional transfer-messenger RNA.Biochimie · 2011Review
- The role of SmpB and the ribosomal decoding center in licensing tmRNA entry into stalled ribosomes.RNA (New York, N.Y.) · 2011Article
- tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation.Journal of nucleic acids · 2011Article
- tmRNA-SmpB: a journey to the centre of the bacterial ribosome.The EMBO journal · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To add a tag-peptide for degradation to the nascent polypeptide in a stalled ribosome, an unusual translation called trans-translation is facilitated by transfer-messenger RNA (tmRNA) having an upper half of the tRNA structure and the sequence encoding the tag-peptide except the first alanine. During this event, tmRNA enters the vacant A-site of the stalled ribosome without a codon-anticodon interaction, but with a protein factor SmpB. Here, we studied the sites and modes of binding of SmpB to the ribosome by directed hydroxyl radical probing from Fe(II) tethered to SmpB variants. It revealed two SmpB-binding sites, A-site and P-site, on the ribosome. Each SmpB can be superimposed on the lower half of tRNA behaving in translation. The sites of cleavages from Fe(II) tethered to the C-terminal residues of A-site SmpB are aligned along the mRNA path towards the downstream tunnel, while those of P-site SmpB are found almost exclusively around the region of the codon-anticodon interaction in the P-site. We propose a new model of trans-translation in that the C-terminal tail of SmpB initially recognizes the decoding region and the mRNA path free of mRNA by mimicking mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.