ArticlePloS one2007
Conformational reorganization of the SARS coronavirus spike following receptor binding: implications for membrane fusion.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 59 citations in OpenAlex.
- Characterization and immunogenicity assessment of MERS-CoV pre-fusion spike trimeric oligomers as vaccine immunogen.Human vaccines & immunotherapeutics · 2024Article
- Structural Framework for Analysis of CD4+ T-Cell Epitope Dominance in Viral Fusion Proteins.Biochemistry · 2023Review
- Roles of host proteases in the entry of SARS-CoV-2.Animal diseases · 2023Review
- Ionizing Radiation Technologies for Vaccine Development - A Mini Review.Frontiers in immunology · 2022Review
- Spike residue 403 affects binding of coronavirus spikes to human ACE2.Nature communications · 2021Article
- Mechanical activation of spike fosters SARS-CoV-2 viral infection.Cell research · 2021Article
- Effect of clinical isolate or cleavage site mutations in the SARS-CoV-2 spike protein on protein stability, cleavage, and cell-cell fusion.The Journal of biological chemistry · 2021Article
- Effect of mutations in the SARS-CoV-2 spike protein on protein stability, cleavage, and cell-cell fusion function.bioRxiv : the preprint server for biology · 2021Article
- Domains and Functions of Spike Protein in Sars-Cov-2 in the Context of Vaccine Design.Viruses · 2021Review
- Cryo-EM analysis of the HCoV-229E spike glycoprotein reveals dynamic prefusion conformational changes.Nature communications · 2021Article
- A graph-based approach identifies dynamic H-bond communication networks in spike protein S of SARS-CoV-2.Journal of structural biology · 2020Article
- ACE2, the Receptor that Enables Infection by SARS-CoV-2: Biochemistry, Structure, Allostery and Evaluation of the Potential Development of ACE2 Modulators.ChemMedChem · 2020Review
- COVID-19: Risk groups, mechanistic insights and challenges.International journal of clinical practice · 2020Article
- Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein.Nature communications · 2020Article
- Highly pathogenic coronaviruses: thrusting vaccine development in the spotlight.Acta pharmaceutica Sinica. B · 2020Review
- Structural insights into coronavirus entry.Advances in virus research · 2019Article
- Viral metagenomics, protein structure, and reverse genetics: Key strategies for investigating coronaviruses.Virology · 2018Review
- Crucial steps in the structure determination of a coronavirus spike glycoprotein using cryo-electron microscopy.Protein science : a publication of the Protein Society · 2017Article
- Structure, Function, and Evolution of Coronavirus Spike Proteins.Annual review of virology · 2016Review
- Supramolecular Architecture of the Coronavirus Particle.Advances in virus research · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SARS coronavirus (SARS-CoV) spike is the largest known viral spike molecule, and shares a similar function with all class 1 viral fusion proteins. Previous structural studies of membrane fusion proteins have largely used crystallography of static molecular fragments, in isolation of their transmembrane domains. In this study we have produced purified, irradiated SARS-CoV virions that retain their morphology, and are fusogenic in cell culture. We used cryo-electron microscopy and image processing to investigate conformational changes that occur in the entire spike of intact virions when they bind to the viral receptor, angiotensin-converting enzyme 2 (ACE2). We have shown that ACE2 binding results in structural changes that appear to be the initial step in viral membrane fusion, and precisely localized the receptor-binding and fusion core domains within the entire spike. Furthermore, our results show that receptor binding and subsequent membrane fusion are distinct steps, and that each spike can bind up to three ACE2 molecules. The SARS-CoV spike provides an ideal model system to study receptor binding and membrane fusion in the native state, employing cryo-electron microscopy and single-particle image analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.