ArticlePloS one2007
High speed two-photon imaging of calcium dynamics in dendritic spines: consequences for spine calcium kinetics and buffer capacity.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 61 citations in OpenAlex.
- Deep learning-assisted mapping of dendritic spines using sequential 2D two-photon calcium imaging.iScience · 2026Article
- All-optical mapping of CaCell calcium · 2025Article
- Magnetic Stimulation as a Therapeutic Approach for Brain Modulation and Repair: Underlying Molecular and Cellular Mechanisms.International journal of molecular sciences · 2023Review
- Dendritic spine morphology regulates calcium-dependent synaptic weight change.The Journal of general physiology · 2022Article
- A calcium-based plasticity model for predicting long-term potentiation and depression in the neocortex.Nature communications · 2022Article
- Synaptic Components, Function and Modulation Characterized by GCaMP6f CaFrontiers in physiology · 2021Article
- Article
- CaeLife · 2020Article
- Automated Metadata Suggestion During Repository Submission.Neuroinformatics · 2019Article
- Caldendrin and Calneurons-EF-Hand CaM-Like Calcium Sensors With Unique Features and Specialized Neuronal Functions.Frontiers in molecular neuroscience · 2019Review
- Active dendrites regulate the spatiotemporal spread of signaling microdomains.PLoS computational biology · 2018Article
- Article
- Inferring Neuronal Dynamics from Calcium Imaging Data Using Biophysical Models and Bayesian Inference.PLoS computational biology · 2016Article
- Modeling spontaneous activity across an excitable epithelium: Support for a coordination scenario of early neural evolution.Frontiers in computational neuroscience · 2015Article
- Buffer mobility and the regulation of neuronal calcium domains.Frontiers in cellular neuroscience · 2015Review
- Article
- Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
- Stochastic calcium mechanisms cause dendritic calcium spike variability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Visualization of fast calcium oscillations in the parafascicular nucleus.Pflugers Archiv : European journal of physiology · 2013Article
- Quantitative interactions between the A-type K+ current and inositol trisphosphate receptors regulate intraneuronal Ca2+ waves and synaptic plasticity.The Journal of physiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid calcium concentration changes in postsynaptic structures are crucial for synaptic plasticity. Thus far, the determinants of postsynaptic calcium dynamics have been studied predominantly based on the decay kinetics of calcium transients. Calcium rise times in spines in response to single action potentials (AP) are almost never measured due to technical limitations, but they could be crucial for synaptic plasticity. With high-speed, precisely-targeted, two-photon point imaging we measured both calcium rise and decay kinetics in spines and secondary dendrites in neocortical pyramidal neurons. We found that both rise and decay kinetics of changes in calcium-indicator fluorescence are about twice as fast in spines. During AP trains, spine calcium changes follow each AP, but not in dendrites. Apart from the higher surface-to-volume ratio (SVR), we observed that neocortical dendritic spines have a markedly smaller endogenous buffer capacity with respect to their parental dendrites. Calcium influx time course and calcium extrusion rate were both in the same range for spines and dendrites when fitted with a dynamic multi-compartment model that included calcium binding kinetics and diffusion. In a subsequent analysis we used this model to investigate which parameters are critical determinants in spine calcium dynamics. The model confirmed the experimental findings: a higher SVR is not sufficient by itself to explain the faster rise time kinetics in spines, but only when paired with a lower buffer capacity in spines. Simulations at zero calcium-dye conditions show that calmodulin is more efficiently activated in spines, which indicates that spine morphology and buffering conditions in neocortical spines favor synaptic plasticity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.