ArticleMolecular genetics and genomics : MGG2008
MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice.
Article in Molecular genetics and genomics : MGG, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed.
- From wild shrub to cultivated host: a genetic roadmap for enhancing lac production inFrontiers in plant science · 2026Review
- Characterization of phenotypic and genetic diversity in sodium azide-induced mutant populations in oat (Avena sativa).BMC plant biology · 2025Article
- Finger millet: a hero in the making to combat food insecurity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- MiRiQ Database: A Platform for In Silico Rice Mutant Screening.Plant & cell physiology · 2024Article
- Chromosomal Location ofPlants (Basel, Switzerland) · 2023Article
- Comprehensive effects of heavy-ion beam irradiation on sweet potato (Plant biotechnology (Tokyo, Japan) · 2022Article
- Whole-Genome Sequencing of Rice Mutant Library Members Induced by N-Methyl-N-Nitrosourea Mutagenesis of Fertilized Egg Cells.Rice (New York, N.Y.) · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Collection, preservation and distribution ofBreeding science · 2021Article
- Applications of Multi-Omics Technologies for Crop Improvement.Frontiers in plant science · 2021Review
- Mutation resource of Samba Mahsuri revealed the presence of high extent of variations among key traits for rice improvement.PloS one · 2021Article
- Deployment of Genetic and Genomic Tools Toward Gaining a Better Understanding of Rice-Frontiers in plant science · 2020Review
- Effective identification of soybean candidate genes involved in resistance to soybean cyst nematode via direct whole genome re-sequencing of two segregating mutants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Generation and Characterization of a Foxtail Millet (Frontiers in plant science · 2019Article
- Mutagenesis in Rice: The Basis for Breeding a New Super Plant.Frontiers in plant science · 2019Review
- Novel Informatic Tools to Support Functional Annotation of the Durum Wheat Genome.Frontiers in plant science · 2019Article
- TheFrontiers in plant science · 2019Article
- High-resolution mapping and characterization ofBreeding science · 2018Article
- Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice.Journal of experimental botany · 2018Article
- Identification of induced mutations in hexaploid wheat genome using exome capture assay.PloS one · 2018Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutant populations are indispensable genetic resources for functional genomics in all organisms. However, suitable rice mutant populations, induced either by chemicals or irradiation still have been rarely developed to date. To produce mutant pools and to launch a search system for rice gene mutations, we developed mutant populations of Oryza sativa japonica cv. Taichung 65, by treating single zygotic cells with N-methyl-N-nitrosourea (MNU). Mutagenesis in single zygotes can create mutations at a high frequency and rarely forms chimeric plants. A modified TILLING system using non-labeled primers and fast capillary gel electrophoresis was applied for high-throughput detection of single nucleotide substitution mutations. The mutation rate of an M(2) mutant population was calculated as 7.4 x 10(-6) per nucleotide representing one mutation in every 135 kb genome sequence. One can expect 7.4 single nucleotide substitution mutations in every 1 kb of gene region when using 1,000 M(2) mutant lines. The mutations were very evenly distributed over the regions examined. These results indicate that our rice mutant population generated by MNU-mutagenesis could be a promising resource for identifying mutations in any gene of rice. The modified TILLING method also proved very efficient and convenient in screening the mutant population.
Indexed as
Identifiers
17952471What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.