ArticlePflugers Archiv : European journal of physiology2008
H-ras transformation sensitizes volume-activated anion channels and increases migratory activity of NIH3T3 fibroblasts.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Four phase 1 trials to evaluate the safety and pharmacokinetic profile of single and repeated dosing of SCO-101 in adult male and female volunteers.Basic & clinical pharmacology & toxicology · 2020Trial
- pH-regulated single cell migration.Pflugers Archiv : European journal of physiology · 2024Review
- LRRC8A is essential for volume-regulated anion channel in smooth muscle cells contributing to cerebrovascular remodeling during hypertension.Cell proliferation · 2021Article
- From Pinocytosis to Methuosis-Fluid Consumption as a Risk Factor for Cell Death.Frontiers in cell and developmental biology · 2021Review
- NPPB prevents postoperative peritoneal adhesion formation by blocking volume-activated ClNaunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Binding of the protein ICln to α-integrin contributes to the activation of IClScientific reports · 2019Article
- The NaThe Journal of physiology · 2019Article
- VRACs and other ion channels and transporters in the regulation of cell volume and beyond.Nature reviews. Molecular cell biology · 2016Review
- Biophysics and Physiology of the Volume-Regulated Anion Channel (VRAC)/Volume-Sensitive Outwardly Rectifying Anion Channel (VSOR).Pflugers Archiv : European journal of physiology · 2016Review
- Activation of ATP secretion via volume-regulated anion channels by sphingosine-1-phosphate in RAW macrophages.Pflugers Archiv : European journal of physiology · 2015Article
- Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance.Channels (Austin, Tex.) · 2015Review
- Ion channels and transporters in tumour cell migration and invasion.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration.Pflugers Archiv : European journal of physiology · 2013Article
- Regulation of bradykinin-induced activation of volume-sensitive outwardly rectifying anion channels by Ca2+ nanodomains in mouse astrocytes.The Journal of physiology · 2011Article
- Directional cell migration and chemotaxis in wound healing response to PDGF-AA are coordinated by the primary cilium in fibroblasts.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2010Article
- Protons make tumor cells move like clockwork.Pflugers Archiv : European journal of physiology · 2009Review
- Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices.Experimental cell research · 2008Article
- Activation of cell migration with fibroblast growth factor-2 requires calcium-sensitive potassium channels.Pflugers Archiv : European journal of physiology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the H-ras oncogene increases the migratory activity of many cell types and thereby contributes to the metastatic behavior of tumor cells. Other studies point to an involvement of volume-activated anion channels (VRAC) in (tumor) cell migration. In this paper, we tested whether VRACs are required for the stimulation of cell migration upon expression of the H-ras oncogene. We compared VRAC activation and migration of wild-type and H-ras-transformed NIH3T3 fibroblasts by means of patch-clamp techniques and time-lapse video microscopy. Both cell types achieve the same degree of VRAC activation upon maximal stimulation, induced by reducing extracellular osmolarity from 300 to 190 mOsm/l. However, upon physiologically relevant reductions in extracellular osmolarity (275 mOsm/l), the level of VRAC activation is almost three times higher in H-ras-transformed compared to wild-type fibroblasts. This increase in VRAC sensitivity is accompanied by increased migratory activity of H-ras fibroblasts. Moreover, the high-affinity VRAC blocker NS3728 inhibits migration of H-ras fibroblasts dose-dependently by up to about 60%, whereas migration of wild-type fibroblasts is reduced by only about 35%. Consistent with higher VRAC activity in H-ras than in wild-type fibroblasts, more VRAC blocker is needed to achieve a comparable degree of inhibition of migration. We suggest that H-ras modulates the volume set point of VRAC and thus facilitates transient changes of cell volume required for faster cell migration.
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