ReviewMethods in molecular biology (Clifton, N.J.)2007
Selection for 3'-end triplets for polymerase chain reaction primers.
Review in Methods in molecular biology (Clifton, N.J.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Effective primer design for genotype and subtype detection of highly divergent viruses in large scale genome datasets.BMC bioinformatics · 2025Article
- A tool for design of primers for microRNA-specific quantitative RT-qPCR.BMC bioinformatics · 2014Article
- Article
- Integrating macro-microscopy, DNA barcoding and HPTLC for quality assessment of berberine containing botanicals traded as Maramanjal/Daruharidra.Journal of Ayurveda and integrative medicineArticle
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1 author.
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Abstract
Primer extension by thermostable DNA polymerase in PCR starts from the 3'-end of a primer. If the PCR starting process fails, the entire PCR fails. Primer sequences at the 3'-end often interfere with success in PCR experiments. Over 2000 primer sequences from successful PCR experiments used with varieties of templates and conditions were analyzed for finding frequencies of the 3'-end triplets. This chapter discusses a trend in 3'-end triplet frequencies in primers used in successful PCR experiments and proposes requirements for the 3'-end of a primer. Finally, a method to select primers with the best 3'-end triplets is introduced based on the 3'-end analysis result.
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