ArticleMolecular & cellular proteomics : MCP2008
Functional dissection of a HECT ubiquitin E3 ligase.
Article in Molecular & cellular proteomics : MCP, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 78 citations in OpenAlex.
- Enzymatic analysis of WWP2 E3 ubiquitin ligase using protein microarrays identifies autophagy-related substrates.The Journal of biological chemistry · 2022Article
- Robust and facile purification of full-length, untagged human Nedd4 as a recombinant protein from Escherichia coli.Protein expression and purification · 2020Article
- Developments and Applications of Functional Protein Microarrays.Molecular & cellular proteomics : MCP · 2020Review
- Remodeling Membrane Binding by Mono-Ubiquitylation.Biomolecules · 2019Article
- Proteome-wide Tyrosine Phosphorylation Analysis Reveals Dysregulated Signaling Pathways in Ovarian Tumors.Molecular & cellular proteomics : MCP · 2019Article
- Ubiquitination as a Key Regulator of Endosomal Signaling by GPCRs.Frontiers in cell and developmental biology · 2019Review
- Global Identification of Small Ubiquitin-related Modifier (SUMO) Substrates Reveals Crosstalk between SUMOylation and Phosphorylation Promotes Cell Migration.Molecular & cellular proteomics : MCP · 2018Article
- Deubiquitinating enzymes Ubp2 and Ubp15 regulate endocytosis by limiting ubiquitination and degradation of ARTs.Molecular biology of the cell · 2017Article
- Protein Array-based Approaches for Biomarker Discovery in Cancer.Genomics, proteomics & bioinformatics · 2017Review
- A Human Proteome Array Approach to Identifying Key Host Proteins Targeted byMolecular & cellular proteomics : MCP · 2017Article
- Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.The EMBO journal · 2017Article
- Systematic approaches to identify E3 ligase substrates.The Biochemical journal · 2016Review
- Gsk3β and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease.The Biochemical journal · 2016Article
- Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism.Nature communications · 2016Article
- The Potentials and Pitfalls of Microarrays in Neglected Tropical Diseases: A Focus on Human Filarial Infections.Microarrays (Basel, Switzerland) · 2016Review
- Review
- The F-box protein FBXO25 promotes the proteasome-dependent degradation of ELK-1 protein.The Journal of biological chemistry · 2013Article
- Overview of protein microarrays.Current protocols in protein science · 2013Review
- Interactome mapping: using protein microarray technology to reconstruct diverse protein networks.Genomics, proteomics & bioinformatics · 2013Review
- Proteomic identification of protein ubiquitination events.Biotechnology & genetic engineering reviews · 2013Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Ubiquitination is one of the most prevalent protein post-translational modifications in eukaryotes, and its malfunction is associated with a variety of human diseases. Despite the significance of this process, the molecular mechanisms that govern the regulation of ubiquitination remain largely unknown. Here we used a combination of yeast proteome chip assays, genetic screening, and in vitro/in vivo biochemical analyses to identify and characterize eight novel in vivo substrates of the ubiquitinating enzyme Rsp5, a homolog of the human ubiquitin-ligating enzyme Nedd4, in yeast. Our analysis of the effects of a deubiquitinating enzyme, Ubp2, demonstrated that an accumulation of Lys-63-linked polyubiquitin chains results in processed forms of two substrates, Sla1 and Ygr068c. Finally we showed that the localization of another newly identified substrate, Rnr2, is Rsp5-dependent. We believe that our approach constitutes a paradigm for the functional dissection of an enzyme with pleiotropic effects.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.