ArticleProceedings of the National Academy of Sciences of the United States of America2007
Potential for hydrogen production with inducible chloroplast gene expression in Chlamydomonas.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 207 citations in OpenAlex.
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Metabolic Engineering of Microalgae for Biofuel Production.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Regulation of nucleus-encoded trans-acting factors allows orthogonal fine-tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii.Plant biotechnology journal · 2025Article
- Biomass-Based Hydrogen Extraction and Accompanying Hazards-Review.Molecules (Basel, Switzerland) · 2025Review
- Light-Driven HPlants (Basel, Switzerland) · 2024Review
- Applications of Synthetic Pentatricopeptide Repeat Proteins.Plant & cell physiology · 2024Review
- An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression.Nucleic acids research · 2023Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast.Bioresources and bioprocessing · 2022Review
- Synthetic biology for improved hydrogen production in Chlamydomonas reinhardtii.Microbial biotechnology · 2022Review
- Overcoming Poor Transgene Expression in the Wild-TypeMicroorganisms · 2022Article
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- The Algal Chloroplast as a Testbed for Synthetic Biology Designs Aimed at Radically Rewiring Plant Metabolism.Frontiers in plant science · 2021Review
- Mechanistic insights into pH-dependent HBiotechnology for biofuels · 2020Article
- Validated Nuclear-Based Transgene Expression Regulated by the Fea1 Iron-Responsive Promoter in the Green Alga Chlamydomonas reinhardtii.Molecular biotechnology · 2019Article
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory.World journal of microbiology & biotechnology · 2018Review
- CITRIC: cold-inducible translational readthrough in the chloroplast of Chlamydomonas reinhardtii using a novel temperature-sensitive transfer RNA.Microbial cell factories · 2018Article
- Article
- Water-splitting-based, sustainable and efficient HBiotechnology for biofuels · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An inducible chloroplast gene expression system was developed in Chlamydomonas reinhardtii by taking advantage of the properties of the copper-sensitive cytochrome c(6) promoter and of the nucleus-encoded Nac2 chloroplast protein. This protein is specifically required for the stable accumulation of the chloroplast psbD RNA and acts on its 5' UTR. A construct containing the Nac2 coding sequence fused to the cytochrome c(6) promoter was introduced into the nac2-26 mutant strain deficient in Nac2. In this transformant, psbD is expressed in copper-depleted but not in copper-replete medium. Because psbD encodes the D2 reaction center polypeptide of photosystem II (PSII), the repression of psbD leads to the loss of PSII. We have tested this system for hydrogen production. Upon addition of copper to cells pregrown in copper-deficient medium, PSII levels declined to a level at which oxygen consumption by respiration exceeded oxygen evolution by PSII. The resulting anaerobic conditions led to the induction of hydrogenase activity. Because the Cyc6 promoter is also induced under anaerobic conditions, this system opens possibilities for sustained cycling hydrogen production. Moreover, this inducible gene expression system is applicable to any chloroplast gene by replacing its 5' UTR with the psbD 5' UTR in the same genetic background. To make these strains phototrophic, the 5' UTR of the psbD gene was replaced by the petA 5' UTR. As an example, we show that the reporter gene aadA driven by the psbD 5' UTR confers resistance to spectinomycin in the absence of copper and sensitivity in its presence in the culture medium.
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