ArticleJournal of bacteriology2008
Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 125 citations in OpenAlex.
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- Development and Application of SRCA for Rapid Detection of Pseudomonas aeruginosa in Bottled Drinking Water.Molecular biotechnology · 2026Article
- Algal Betaine Triggers Bacterial Hydrogen Peroxide Production that Promotes Algal Demise.Nature communications · 2026Article
- Structural basis for EtfD-mediated coupling of β-oxidation and the respiratory chain in mycobacteria.The EMBO journal · 2026Article
- A choline-sensing regulator coordinates metabolic adaptation and pathogenesis inScience advances · 2025Article
- Methionine synthesis and glycine betaine demethylation are intricately intertwined in cosmopolitan marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Dual roles of glycine betaine, dimethylglycine, and sarcosine as osmoprotectants and nutrient sources forApplied and environmental microbiology · 2025Article
- Glycine betaine metabolism is enabled inApplied and environmental microbiology · 2024Article
- Enhanced catabolism of glycine betaine and derivatives provides improved osmotic stress protection inApplied and environmental microbiology · 2024Article
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- Choline degradation inJournal of bacteriology · 2024Article
- In-depth genome and pan-genome analysis of a metal-resistant bacteriumFrontiers in microbiology · 2023Article
- Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome.Journal of microbiology (Seoul, Korea) · 2022Article
- Glycine betaine uptake and metabolism in marine microbial communities.Environmental microbiology · 2022Article
- Metagenomics indicate that public health risk may be higher from flooding following dry versus rainy periods.Environmental microbiology reports · 2022Article
- Interactions between strains govern the eco-evolutionary dynamics of microbial communities.eLife · 2022Article
- Transcriptome Analysis to Understand Salt Stress Regulation Mechanism ofFrontiers in microbiology · 2022Article
- Heterotrophic Sulfur Oxidation ofFrontiers in microbiology · 2022Article
- Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential ofFrontiers in microbiology · 2022Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Glycine betaine (GB), which occurs freely in the environment and is an intermediate in the catabolism of choline and carnitine, can serve as a sole source of carbon or nitrogen in Pseudomonas aeruginosa. Twelve mutants defective in growth on GB as the sole carbon source were identified through a genetic screen of a nonredundant PA14 transposon mutant library. Further growth experiments showed that strains with mutations in two genes, gbcA (PA5410) and gbcB (PA5411), were capable of growth on dimethylglycine (DMG), a catabolic product of GB, but not on GB itself. Subsequent nuclear magnetic resonance (NMR) experiments with 1,2-(13)C-labeled choline indicated that these genes are necessary for conversion of GB to DMG. Similar experiments showed that strains with mutations in the dgcAB (PA5398-PA5399) genes, which exhibit homology to genes that encode other enzymes with demethylase activity, are required for the conversion of DMG to sarcosine. Mutant analyses and (13)C NMR studies also confirmed that the soxBDAG genes, predicted to encode a sarcosine oxidase, are required for sarcosine catabolism. Our screen also identified a predicted AraC family transcriptional regulator, encoded by gbdR (PA5380), that is required for growth on GB and DMG and for the induction of gbcA, gbcB, and dgcAB in response to GB or DMG. Mutants defective in the previously described gbt gene (PA3082) grew on GB with kinetics similar to those of the wild type in both the PAO1 and PA14 strain backgrounds. These studies provided important insight into both the mechanism and the regulation of the catabolism of GB in P. aeruginosa.
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