ArticleBiophysical journal2008
Productive hemifusion intermediates in fast vesicle fusion driven by neuronal SNAREs.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- A hemifused complex is the hub in a network of pathways to membrane fusion.Biophysical journal · 2023Article
- Synaptic Vesicles Having Large Contact Areas with the Presynaptic Membrane are Preferentially Hemifused at Active Zones of Frog Neuromuscular Junctions Fixed during Synaptic Activity.International journal of molecular sciences · 2019Review
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- SNARE-Mediated Single-Vesicle Fusion Events with Supported and Freestanding Lipid Membranes.Biophysical journal · 2017Article
- Direct observation of intermediate states in model membrane fusion.Scientific reports · 2016Article
- Direct quantitative detection of Doc2b-induced hemifusion in optically trapped membranes.Nature communications · 2015Article
- Reconstituting SNARE-mediated membrane fusion at the single liposome level.Methods in cell biology · 2015Article
- Individual vesicle fusion events mediated by lipid-anchored DNA.Biophysical journal · 2013Article
- Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion.eLife · 2012Article
- Adhesion energy can regulate vesicle fusion and stabilize partially fused states.Journal of the Royal Society, Interface · 2012Article
- Intermembrane docking reactions are regulated by membrane curvature.Biophysical journal · 2011Article
- Docking and fast fusion of synaptobrevin vesicles depends on the lipid compositions of the vesicle and the acceptor SNARE complex-containing target membrane.Biophysical journal · 2010Article
- Model lipid bilayer with facile diffusion of lipids and integral membrane proteins.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Atomic-resolution simulations predict a transition state for vesicle fusion defined by contact of a few lipid tails.PLoS computational biology · 2010Article
- Direct visualization of large and protein-free hemifusion diaphragms.Biophysical journal · 2010Article
- Lipid mixing and content release in single-vesicle, SNARE-driven fusion assay with 1-5 ms resolution.Biophysical journal · 2009Article
- Fusion step-specific influence of cholesterol on SNARE-mediated membrane fusion.Biophysical journal · 2009Article
- Single-molecule studies of the neuronal SNARE fusion machinery.Annual review of biochemistry · 2009Review
- The fusion pores of Ca2+ -triggered exocytosis.Nature structural & molecular biology · 2008Review
- Mechanics of membrane fusion.Nature structural & molecular biology · 2008Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
An in vitro fusion assay uses fluorescence microscopy of labeled lipids to monitor single v-SNARE vesicle docking and fusion events on a planar lipid bilayer containing t-SNAREs. For vesicles and bilayer comprising phosphatidylcholine (POPC, 84-85% by mol) and phosphatidylserine (DOPS, 15% by mol), previous work demonstrated prompt, full fusion (tau(fus) = 25 ms). Substitution of 20-60% phosphatidylethanolamine (DOPE) for phosphatidylcholine in the v-SNARE vesicle with either 0 or 20% DOPE included in the t-SNARE bilayer gives rise to hemifusion events. Labeled lipids diffuse into the planar bilayer as two temporally distinct waves, presumably hemifusion of the outer leaflet followed by inner leaflet (core) fusion. The fusion kinetics with DOPE is markedly heterogeneous. Some vesicle/docking site pairs exhibit prompt, full fusion while others exhibit hemifusion. Hemifusion events are roughly half productive (leading to subsequent core fusion within 20 s) and half dead-end. In qualitative accord with expectations from studies of protein-free vesicle-vesicle fusion, the hemifusion rate k(hemi) is 15-20 times faster than the core fusion rate k(core), and the fraction of hemifusion events increases with increasing percentage of DOPE. This suggests similar underlying molecular pathways for protein-free and neuronal SNARE-driven fusion. Removal of phosphatidylserine from the v-SNARE vesicle has no effect on docking or fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.