ArticleMolecular and biochemical parasitology2007
Evidence for a capping enzyme with specificity for the trypanosome spliced leader RNA.
Article in Molecular and biochemical parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- The trypanosome mRNA decapping enzyme ALPH1 prefers caps without m7G methylation and produces diphosphate RNA.Nucleic acids research · 2026Article
- Trypanosome mRNA recapping is triggered by hypermethylation originating from cap 4.Nucleic acids research · 2024Article
- LeishIF3d is a non-canonical cap-binding protein inFrontiers in molecular biosciences · 2023Article
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Comparative proteomics of the two T. brucei PABPs suggests that PABP2 controls bulk mRNA.PLoS neglected tropical diseases · 2018Article
- The Role of Cytoplasmic mRNA Cap-Binding Protein Complexes in Trypanosoma brucei and Other Trypanosomatids.Pathogens (Basel, Switzerland) · 2017Review
- The messenger RNA decapping and recapping pathway in Trypanosoma.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Design and synthesis of a series of truncated neplanocin fleximers.Molecules (Basel, Switzerland) · 2014Article
- eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei.RNA (New York, N.Y.) · 2014Article
- Trypanosoma brucei translation initiation factor homolog EIF4E6 forms a tripartite cytosolic complex with EIF4G5 and a capping enzyme homolog.Eukaryotic cell · 2014Article
- Two splicing factors carrying serine-arginine motifs, TSR1 and TSR1IP, regulate splicing, mRNA stability, and rRNA processing in Trypanosoma brucei.RNA biology · 2014Article
- Basal splicing factors regulate the stability of mature mRNAs in trypanosomes.The Journal of biological chemistry · 2013Article
- Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate.Journal of cell science · 2012Article
- A metazoan/plant-like capping enzyme and cap modified nucleotides in the unicellular eukaryote Trichomonas vaginalis.PLoS pathogens · 2010Article
- Hypermethylated cap 4 maximizes Trypanosoma brucei translation.Molecular microbiology · 2009Article
- Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex.Molecular and cellular biology · 2009Article
- Trypanosome spliced-leader-associated RNA (SLA1) localization and implications for spliced-leader RNA biogenesis.Eukaryotic cell · 2009Article
- The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis.Molecular microbiology · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Capping of the pre-mRNA 5' end by addition a monomethylated guanosine cap (m(7)G) is an essential and the earliest modification in the biogenesis of mRNA. The reaction is catalyzed by three enzymes: triphosphatase, guanylyltransferase, and (guanine N-7) methyltransferase. Whereas this modification occurs co-transcriptionally in most eukaryotic organisms, trypanosomatid protozoa mRNAs acquire the m(7)G cap by trans-splicing, which entails the transfer of the capped spliced leader (SL) from the SL RNA to the mRNA. Intriguingly, the genomes of all trypanosomatid protozoa sequenced to date possess two distinct proteins with the signature motifs of guanylyltransferases: TbCGM1 and the previously characterized TbCE1. Here we provide biochemical evidence that TbCgm1 is a capping enzyme. Whereas RNAi-induced downregulation of TbCe1 had no phenotypic consequences, we found that TbCGM1 is essential for trypanosome viability and is required for SL RNA capping. Furthermore, consistent with co-transcriptional addition of the m(7)G cap, chromatin immunoprecipitation revealed recruitment of TbCgm1 to the SL RNA genes.
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