ArticleGenetics2007
Sex-specific splicing of the honeybee doublesex gene reveals 300 million years of evolution at the bottom of the insect sex-determination pathway.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 127 citations in OpenAlex.
- Comprehensive Genome-Wide Analysis of Dmrt Transcription Factors Reveals Their Roles in Sexual Dimorphism inBiology · 2025Article
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- A Conserved Somatic Sex Determination Cascade Instructs Trait-Specific Sexual Dimorphism in Horned Dung Beetles.Evolution & development · 2025Article
- Article
- Candidate target genes of the male-specific expressed Doublesex in the termite Reticulitermes speratus.PloS one · 2024Article
- Recognition of polymorphic Csd proteins determines sex in the honeybee.Science advances · 2023Article
- The draft genome sequence of the Japanese rhinoceros beetle Trypoxylus dichotomus septentrionalis towards an understanding of horn formation.Scientific reports · 2023Article
- Regulators of male and female sexual development are critical for the transmission of a malaria parasite.Cell host & microbe · 2023Article
- CRISPR/Cas9-Mediated Mutagenesis of Sex-SpecificCells · 2022Article
- CRISPR/Cas9 mediated sex-ratio distortion by sex specific gene editing inSaudi journal of biological sciences · 2022Article
- Manipulating Insect Sex Determination Pathways for Genetic Pest Management: Opportunities and Challenges.Frontiers in bioengineering and biotechnology · 2022Review
- Genome annotation with long RNA reads reveals new patterns of gene expression and improves single-cell analyses in an ant brain.BMC biology · 2021Article
- Effects of Functional Depletion ofInsects · 2021Article
- Transcriptional Profiles of Diploid MutantInsects · 2021Article
- Article
- Chemoreceptor Diversity in Apoid Wasps and Its Reduction during the Evolution of the Pollen-Collecting Lifestyle of Bees (Hymenoptera: Apoidea).Genome biology and evolution · 2021Article
- Spliceosomal Protein GeneInternational journal of molecular sciences · 2020Article
- Genome of the Parasitoid Wasp Diachasma alloeum, an Emerging Model for Ecological Speciation and Transitions to Asexual Reproduction.Genome biology and evolution · 2019Article
- Hemimetabolous insects elucidate the origin of sexual development via alternative splicing.eLife · 2019Article
- A genetic switch for worker nutrition-mediated traits in honeybees.PLoS biology · 2019Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex-determination mechanisms vary greatly among taxa. It has been proposed that genetic sex-determination pathways evolve in reverse order from the final step in the pathway to the first step. Consistent with this hypothesis, doublesex (dsx), the most downstream gene in the Drosophila sex-determination cascade that determines most sexual phenotypes also determines sex in other dipterans and the silk moth, while the upstream genes vary among these species. However, it is unknown when dsx was recruited to the sex-determination pathway during insect evolution. Furthermore, sex-specific splicing of dsx, by which dsx determines sex, is different in pattern and mechanism between the moth and the fly, raising an interesting question of how these insects have kept the executor of sex determination while allowing flexibility in the means of execution. To address these questions, here we study the dsx gene of the honeybee Apis mellifera, a member of the most basal lineage of holometabolous insects. We report that honeybee dsx is sex-specifically spliced and that it produces both the fly-type and moth-type splicing forms, indicating that the use of different splicing forms of Dsx in controlling sexual differentiation was present in the common ancestor of holometabolous insects. Our data suggest that in ancestral holometabolous insects the female Dsx form is the default and the male form is generated by suppressing the splicing of the female form. Thus, it is likely that the dsx splicing activator system in flies, where the male form is the default, arose during early dipteran evolution.
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