ArticleNucleic acids research2007
An engineered L-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 71 citations in OpenAlex.
- Arginine: at the crossroads of nitrogen metabolism.The EMBO journal · 2025Review
- Synthetic Gene Circuits for Regulation of Next-Generation Cell-Based Therapeutics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Engineering natural molecule-triggered genetic control systems for tunable gene- and cell-based therapies.Synthetic and systems biotechnology · 2023Article
- Combinatorial protein dimerization enables precise multi-input synthetic computations.Nature chemical biology · 2023Article
- Controlling therapeutic protein expression via inhalation of a butter flavor molecule.Nucleic acids research · 2023Article
- Biomarker-driven feedback control of synthetic biology systems for next-generation personalized medicine.Frontiers in bioengineering and biotechnology · 2022Review
- Intracellular arginine-dependent translation sensor reveals the dynamics of arginine starvation response and resistance in ASS1-negative cells.Cancer & metabolism · 2021Article
- Small-molecule inducible transcriptional control in mammalian cells.Critical reviews in biotechnology · 2020Review
- Review
- Engineering Gene Circuits for Mammalian Cell-Based Applications.Cold Spring Harbor perspectives in biology · 2016Review
- Synthetic dual-input mammalian genetic circuits enable tunable and stringent transcription control by chemical and light.Nucleic acids research · 2016Article
- Cosmetics-triggered percutaneous remote control of transgene expression in mice.Nucleic acids research · 2015Article
- Mammalian synthetic biology: emerging medical applications.Journal of the Royal Society, Interface · 2015Review
- Systematic transfer of prokaryotic sensors and circuits to mammalian cells.ACS synthetic biology · 2014Article
- Antagonistic control of a dual-input mammalian gene switch by food additives.Nucleic acids research · 2014Article
- The food additive vanillic acid controls transgene expression in mammalian cells and mice.Nucleic acids research · 2012Article
- Eukaryotic systems broaden the scope of synthetic biology.The Journal of cell biology · 2009Review
- Controlling transgene expression in subcutaneous implants using a skin lotion containing the apple metabolite phloretin.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Intronically encoded siRNAs improve dynamic range of mammalian gene regulation systems and toggle switch.Nucleic acids research · 2008Article
- Arginase-negative mutants of Arabidopsis exhibit increased nitric oxide signaling in root development.Plant physiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For optimal compatibility with biopharmaceutical manufacturing and gene therapy, heterologous transgene control systems must be responsive to side-effect-free physiologic inducer molecules. The arginine-inducible interaction of the ArgR repressor and the ArgR-specific ARG box, which synchronize arginine import and synthesis in the intracellular human pathogen Chlamydia pneumoniae, was engineered for arginine-regulated transgene (ART) expression in mammalian cells. A synthetic arginine-responsive transactivator (ARG), consisting of ArgR fused to the Herpes simplex VP16 transactivation domain, reversibly adjusted transgene transcription of chimeric ARG box-containing mammalian minimal promoters (P(ART)) in an arginine-inducible manner. Arginine-controlled transgene expression showed rapid induction kinetics in a variety of mammalian cell lines and was adjustable and reversible at concentrations which were compatible with host cell physiology. ART variants containing different transactivation domains, variable spacing between ARG box and minimal promoter and several tandem ARG boxes showed modified regulation performance tailored for specific expression scenarios and cell types. Mice implanted with microencapsulated cells engineered for ART-inducible expression of the human placental secreted alkaline phosphatase (SEAP) exhibited adjustable serum phosphatase levels after treatment with different arginine doses. Using a physiologic inducer, such as the amino acid l-arginine, to control heterologous transgenes in a seamless manner which is devoid of noticeable metabolic interference will foster novel opportunities for precise expression dosing in future gene therapy scenarios as well as the manufacturing of difficult-to-produce protein pharmaceuticals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.