ReviewTrends in biotechnology2007
Diolistics: incorporating fluorescent dyes into biological samples using a gene gun.
Review in Trends in biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Techniques to Render Dendritic Spines Visible in the Microscope.Advances in neurobiology · 2023Article
- Using a Hand-Held Gene Gun for Genetic Transformation of Tetrahymena thermophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Unraveling Individual Differences In The HIV-1 Transgenic Rat: Therapeutic Efficacy Of Methylphenidate.Scientific reports · 2018Article
- Synthesis, characterization and photophysical properties of novel azole derivatives.Journal of fluorescence · 2015Article
- A microarray MEMS device for biolistic delivery of vaccine and drug powders.Human vaccines & immunotherapeutics · 2015Article
- HIV-1 transgenic female rat: synaptodendritic alterations of medium spiny neurons in the nucleus accumbens.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2014Article
- Regioselective biolistic targeting in organotypic brain slices using a modified gene gun.Journal of visualized experiments : JoVE · 2014Article
- Proteolistics: a biolistic method for intracellular delivery of proteins.Transgenic research · 2014Article
- Neutralization of Nogo-A enhances synaptic plasticity in the rodent motor cortex and improves motor learning in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- DiOLISTIC labeling of neurons from rodent and non-human primate brain slices.Journal of visualized experiments : JoVE · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The hand-held gene gun provides a rapid and efficient method of incorporating fluorescent dyes into cells, a technique that is becoming known as diolistics. Transporting fluorescent dyes into cells has, in the past, used predominantly injection or chemical methods. The use of the gene gun, combined with the new generation of fluorescent dyes, circumvents some of the problems of using these methods and also enables the study of cells that have proved difficult traditionally to transfect (e.g. those deep in tissues and/or terminally differentiated); in addition, the use of ion- or metabolite-sensitive dyes provides a route to study cellular mechanisms. Diolistics is also ideal for loading cells with optical nanosensors--nanometre-sized sensors linked to fluorescent probes. Here, we discuss the theoretical considerations of using diolistics, the advantages compared with other methods of inserting dyes into cells and the current uses of the technique, with particular consideration of nanosensors.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.