ArticleExperimental parasitology2008
Strongyloides stercoralis: cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3' UTR.
Article in Experimental parasitology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 66 citations in OpenAlex.
- Neuronally sensed oxygen drives behavior and development in human-infective, skin-penetrating nematodes.bioRxiv : the preprint server for biology · 2026Article
- Transgenesis and targeted mutagenesis in the human-parasitic nematodeFrontiers in parasitology · 2026Review
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- Aptazyme-mediated gene regulation in Strongyloides stercoralis for functional studies of insulin receptor isoform specificity.PLoS pathogens · 2025Article
- Carbon dioxide shapes parasite-host interactions in a human-infective nematode.Current biology : CB · 2025Article
- Microparticle Bombardment as a Method for Transgenesis inmicroPublication biology · 2025Article
- The generation of stable transgenic lines in the human-infective nematode Strongyloides stercoralis.G3 (Bethesda, Md.) · 2024Article
- Carbon dioxide shapes parasite-host interactions in a human-infective nematode.bioRxiv : the preprint server for biology · 2024Article
- A standard workflow for community-driven manual curation ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Lentiviral Transduction-based CRISPR/Cas9 Editing ofCurrent genomics · 2023Article
- Domain definition and preliminary functional exploration of the endonuclease NOBP-1 in Strongyloides stercoralis.Parasites & vectors · 2023Article
- Using newly optimized genetic tools to probe Strongyloides sensory behaviors.Molecular and biochemical parasitology · 2022Review
- The neural basis of heat seeking in a human-infective parasitic worm.Current biology : CB · 2022Article
- Transgenesis in parasitic helminths: a brief history and prospects for the future.Parasites & vectors · 2022Review
- Generating Transgenics and Knockouts in Strongyloides Species by Microinjection.Journal of visualized experiments : JoVE · 2021Article
- Transgenic expression of a T cell epitope in Strongyloides ratti reveals that helminth-specific CD4+ T cells constitute both Th2 and Treg populations.PLoS pathogens · 2021Article
- The role of carbon dioxide in nematode behaviour and physiology.Parasitology · 2020Review
- Recent advances in functional genomics for parasitic nematodes of mammals.The Journal of experimental biology · 2020Review
- Advances in the Molecular and Cellular Biology ofCurrent tropical medicine reports · 2019Article
- The Experimental Infections of the Human Isolate of Strongyloides Stercoralis in a Rodent Model (The Mongolian Gerbil, Meriones Unguiculatus).Pathogens (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Transgenesis is a valuable methodology for studying gene expression patterns and gene function. It has recently become available for research on some parasitic nematodes, including Strongyloides stercoralis. Previously, we described a vector construct, comprising the promoter and 3' UTR of the S. stercoralis gene Ss era-1 that gives expression of GFP in intestinal cells of developing F1 progeny. In the present study, we identified three new S. stercoralis promoters, which, in combination with the Ss era-1 3' UTR, can drive expression of GFP or the red fluorescent protein, mRFPmars, in tissue-specific fashion. These include Ss act-2, which drives expression in body wall muscle cells, Ss gpa-3, which drives expression in amphidial and phasmidial neurons and Ss rps-21, which drives ubiquitous expression in F1 transformants and in the gonads of microinjected P0 female worms. Concomitant microinjection of vectors containing GFP and mRFPmars gave dually transformed F1 progeny, suggesting that these constructs could be used as co-injection markers for other transgenes of interest. We have developed a vector "toolkit" for S. stercoralis including constructs with the Ss era-1 3' UTR and each of the promoters described above.
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Registered trials
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