ArticleLetters in applied microbiology2008
Rapid detection of H5 avian influenza virus by TaqMan-MGB real-time RT-PCR.
Article in Letters in applied microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Advances in Detection Techniques for the H5N1 Avian Influenza Virus.International journal of molecular sciences · 2023Review
- The arrival of highly pathogenic avian influenza viruses H5N8 in Iran through two windows, 2016.Virus genes · 2022Article
- Multiplex one-step Real-time PCR by Taqman-MGB method for rapid detection of pan and H5 subtype avian influenza viruses.PloS one · 2017Article
- A complete molecular diagnostic procedure for applications in surveillance and subtyping of avian influenza virus.BioMed research international · 2014Article
- Development of a real-time reverse-transcription-PCR method for detection of RD114 virus in canine vaccines.Biologicals : journal of the International Association of Biological Standardization · 2011Article
- Article
- Methods for molecular surveillance of influenza.Expert review of anti-infective therapy · 2010Review
- A real-time RT-PCR for detection of clade 1 and 2 H5N1 influenza A virus using locked nucleic acid (LNA) TaqMan probes.Virology journal · 2010Article
- Real-time PCR-based pathotyping of Newcastle disease virus by use of TaqMan minor groove binder probes.Journal of clinical microbiology · 2009Article
- design of multiplexed detection assays for identification of avian influenza a virus subtypes pathogenic to humans by SmartCycler real-time reverse transcription-PCR.Journal of clinical microbiology · 2009Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsReal-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on a TaqMan-minor groove binder (MGB) probe was developed for the rapid detection of avian influenza virus subtype H5. METHODS AND
resultsConserved regions in the haemagglutinin genes of avian influenza viruses subtype H5 served as targets for the primers and TaqMan-MGB probe design. Concentrations of primers and probe were optimized to improve the sensitivity and specificity of the reactions. A plasmid containing the haemagglutinin gene was constructed and in vitro transcribed for a quantitative assay of copy numbers of the target gene. The results revealed that the optimal concentration of primers and probe was 640 and 480 nmol l(-1), respectively. The threshold of 100 copies of target molecules could be detected. The linear range for detection was determined as 10(2) to 10(8) molecules in reaction.
conclusionsIt took less than 3 h to complete the detection from viral RNA extraction, with good sensitivity and repeatability. SIGNIFICANCE AND IMPACT OF THE STUDY: Real-time RT-PCR assay with MGB probe was an effective means for quick and quantitative laboratory detection and monitoring of H5 avian influenza viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.