ArticleMolecular microbiology2007
Role of the Trypanosoma brucei natural cysteine peptidase inhibitor ICP in differentiation and virulence.
Article in Molecular microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Highlights in Cysteine Protease Inhibitors as Antiparasitic Agents Against Trypanosomatids.International journal of molecular sciences · 2026Review
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- Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.PLoS pathogens · 2025Article
- Article
- Cysteine proteases in protozoan parasites.PLoS neglected tropical diseases · 2018Review
- Decoding the network of Trypanosoma brucei proteins that determines sensitivity to apolipoprotein-L1.PLoS pathogens · 2018Article
- Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins.The Journal of biological chemistry · 2015Article
- A cysteine protease inhibitor of plasmodium berghei is essential for exo-erythrocytic development.PLoS pathogens · 2014Article
- Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei.PLoS pathogens · 2014Article
- Article
- Prokaryote-derived protein inhibitors of peptidases: A sketchy occurrence and mostly unknown function.Biochimie · 2010Review
- Exoerythrocytic Plasmodium parasites secrete a cysteine protease inhibitor involved in sporozoite invasion and capable of blocking cell death of host hepatocytes.PLoS pathogens · 2010Article
- Host-parasite interactions in trypanosomiasis: on the way to an antidisease strategy.Infection and immunity · 2009Review
- The cathepsin L of Toxoplasma gondii (TgCPL) and its endogenous macromolecular inhibitor, toxostatin.Molecular and biochemical parasitology · 2009Article
- Influence of parasite encoded inhibitors of serine peptidases in early infection of macrophages with Leishmania major.Cellular microbiology · 2009Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
ICP is a chagasin-family natural tight binding inhibitor of Clan CA, family C1 cysteine peptidases (CPs). We investigated the role of ICP in Trypanosoma brucei by generating bloodstream form ICP-deficient mutants (Deltaicp). A threefold increase in CP activity was detected in lysates of Deltaicp, which was restored to the levels in wild type parasites by re-expression of the gene in the null mutant. Deltaicp displayed slower growth in culture and increased resistance to a trypanocidal synthetic CP inhibitor. More efficient exchange of the variant surface glycoprotein (VSG) to procyclin during differentiation from bloodstream to procyclic form was observed in Deltaicp, a phenotype that was reversed in the presence of synthetic CP inhibitors. Furthermore, we showed that degradation of anti-VSG IgG is abolished when parasites are pretreated with synthetic CP inhibitors, and that parasites lacking ICP degrade IgG more efficiently than wild type. In addition, Deltaicp reached higher parasitemia than wild type parasites in infected mice, suggesting that ICP modulates parasite infectivity. Taken together, these data suggest that CPs of T. brucei bloodstream form play a role in surface coat exchange during differentiation, in the degradation of internalized IgG and in parasite infectivity, and that their function is regulated by ICP.
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