ArticleRNA (New York, N.Y.)2007
3' Terminal oligo U-tract-mediated stimulation of decapping.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 129 citations in OpenAlex.
- MicroRNA-responsive ON-OFF hybrid mRNA switch for precise protein expression control.Molecular therapy. Nucleic acids · 2025Article
- Recycling of Uridylated mRNAs in Starfish Embryos.Biomolecules · 2024Article
- Uridylation regulates mRNA decay directionality in fission yeast.Nature communications · 2024Article
- LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential.Nucleic acids research · 2024Article
- Control of RNA degradation in cell fate decision.Frontiers in cell and developmental biology · 2023Review
- CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.BMC biology · 2022Article
- Targeted RNA editing: novel tools to study post-transcriptional regulation.Molecular cell · 2022Review
- The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis.Nature communications · 2021Article
- The ZSWIM8 ubiquitin ligase mediates target-directed microRNA degradation.Science (New York, N.Y.) · 2020Article
- DIS3L2 and LSm proteins are involved in the surveillance of Sm ring-deficient snRNAs.Nucleic acids research · 2020Article
- UPFront and center in RNA decay: UPF1 in nonsense-mediated mRNA decay and beyond.RNA (New York, N.Y.) · 2019Review
- A programmed wave of uridylation-primed mRNA degradation is essential for meiotic progression and mammalian spermatogenesis.Cell research · 2019Article
- The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy.Molecular cell · 2019Article
- Recognition of Poly(A) RNA through Its Intrinsic Helical Structure.Cold Spring Harbor symposia on quantitative biology · 2019Article
- Structural and molecular mechanisms for the control of eukaryotic 5'-3' mRNA decay.Nature structural & molecular biology · 2018Review
- RNA uridylation and decay in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Role of oligouridylation in normal metabolism and regulated degradation of mammalian histone mRNAs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- The role of 3' end uridylation in RNA metabolism and cellular physiology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Terminal nucleotidyl transferases (TENTs) in mammalian RNA metabolism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- NMD-degradome sequencing reveals ribosome-bound intermediates with 3'-end non-templated nucleotides.Nature structural & molecular biology · 2018Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Decapping is a critical step in the control of gene expression and is regulated by both positive and negative trans factors. Less is known about cis elements that promote decapping. In plants, following microRNA (miRNA)-directed cleavage of an mRNA, a uridine tract can be added onto the exposed 3' end of the resulting 5' fragment, which can promote 5' end decay. We now demonstrate that in mammalian cell extract, addition of five uridine residues to the 3' end of an RNA (U5) promotes decapping relative to an RNA lacking the uridines (U0). Although the decapping stimulation observed in extract required hDcp2, recombinant hDcp2 was unable to support differential decapping of the U0 and U5 RNAs, indicating that the stimulation was likely due to an indirect recruitment of hDcp2 to the RNA. Consistent with the promotion of 5' end decapping by the uridine tract, affinity purification with the U5 RNA revealed the presence of a decapping subcomplex at least consisting of hDcp2, Dcp1a, Edc4, LSm1, and LSm4 that were specifically bound to the U5 RNA but not the U0 RNA. In addition to promoting decapping, the U-tract stabilized the 3' end of the RNA by preventing 3' to 5' exonucleolytic decay to ensure 5' end directional degradation. These data suggest that following post-transcriptional oligo uridylation of an mRNA or mRNA fragment, the U-tract has the capacity to specifically stimulate 5' end decapping to expedite mRNA decay.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.