ArticleGenes & development2007
Trio's Rho-specific GEF domain is the missing Galpha q effector in C. elegans.
Article in Genes & development, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 100 citations in OpenAlex.
- Rho signaling promotes cell excitability and synaptic transmission.bioRxiv : the preprint server for biology · 2025Article
- The Rho GEF Trio functions in contact inhibition of locomotion of neural crest cells by interacting with Ptk7.Development (Cambridge, England) · 2025Article
- Dissecting the Neuronal Contributions of the Lipid Regulator NHR-49 Function in Lifespan and Behavior inLife (Basel, Switzerland) · 2023Article
- Multiple Subthreshold GPCR Signals Combined by the G-Proteins GαThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility.Cell · 2022Article
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- Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Control of Locomotory Behavior ofGenetics · 2020Article
- Structure of the C-terminal guanine nucleotide exchange factor module of Trio in an autoinhibited conformation reveals its oncogenic potential.Science signaling · 2019Article
- Neurotransmitter signaling through heterotrimeric G proteins: insights from studies in C. elegans.WormBook : the online review of C. elegans biology · 2018Review
- Control of Growth Cone Polarity, Microtubule Accumulation, and Protrusion by UNC-6/Netrin and Its Receptors inGenetics · 2018Article
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- Article
- Dopamine negatively modulates the NCA ion channels in C. elegans.PLoS genetics · 2017Article
- The SEK-1 p38 MAP Kinase Pathway Modulates Gq Signaling inG3 (Bethesda, Md.) · 2017Article
- The NCA-1 and NCA-2 Ion Channels Function Downstream of GGenetics · 2017Article
- Activity of theeLife · 2016Article
- Regulation, Signaling, and Physiological Functions of G-Proteins.Journal of molecular biology · 2016Review
- Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon.PloS one · 2016Article
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
Abstract
The Galpha(q) pathway is essential for animal life and is a central pathway for driving locomotion, egg laying, and growth in Caenorhabditis elegans, where it exerts its effects through EGL-8 (phospholipase Cbeta [PLCbeta]) and at least one other effector. To find the missing effector, we performed forward genetic screens to suppress the slow growth and hyperactive behaviors of mutants with an overactive Galpha(q) pathway. Four suppressor mutations disrupted the Rho-specific guanine-nucleotide exchange factor (GEF) domain of UNC-73 (Trio). The mutations produce defects in neuronal function, but not neuronal development, that cause sluggish locomotion similar to animals lacking EGL-8 (PLCbeta). Strains containing null mutations in both EGL-8 (PLCbeta) and UNC-73 (Trio RhoGEF) have strong synthetic phenotypes that phenocopy the arrested growth and near-complete paralysis of Galpha(q)-null mutants. Using cell-based and biochemical assays, we show that activated C. elegans Galpha(q) synergizes with Trio RhoGEF to activate RhoA. Activated Galpha(q) and Trio RhoGEF appear to be part of a signaling complex, because they coimmunoprecipitate when expressed together in cells. Our results show that Trio's Rho-specific GEF domain is a major Galpha(q) effector that, together with PLCbeta, mediates the Galpha(q) signaling that drives the locomotion, egg laying, and growth of the animal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.