ArticleProceedings of the National Academy of Sciences of the United States of America2007
ARF1-mediated actin polymerization produces movement of artificial vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization.EMBO reports · 2026Article
- Mechanistic insights into cargo sorting and export from the Golgi apparatus.Nature reviews. Molecular cell biology · 2025Review
- Unraveling the pathogenic mechanism of a novel filamin a frameshift variant in periventricular nodular heterotopia.Frontiers in pharmacology · 2024Article
- AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in neurodevelopmental disorders.Disease models & mechanisms · 2023Article
- AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in a neurodevelopmental disorder.bioRxiv : the preprint server for biology · 2023Article
- Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO.The Journal of cell biology · 2017Article
- Actin acting at the Golgi.Histochemistry and cell biology · 2013Review
- The small GTPase Arf1 modulates Arp2/3-mediated actin polymerization via PICK1 to regulate synaptic plasticity.Neuron · 2013Article
- Actin polymerization or myosin contraction: two ways to build up cortical tension for symmetry breaking.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Brefeldin A-inhibited ADP-ribosylation factor activator BIG2 regulates cell migration via integrin β1 cycling and actin remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Myosin 1 controls membrane shape by coupling F-Actin to membrane.Bioarchitecture · 2011Article
- Article
- Lamellipodium extension and membrane ruffling require different SNARE-mediated trafficking pathways.BMC cell biology · 2010Article
- Small-molecule p21-activated kinase inhibitor PF-3758309 is a potent inhibitor of oncogenic signaling and tumor growth.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum.Journal of cell science · 2010Article
- Profilin interaction with phosphatidylinositol (4,5)-bisphosphate destabilizes the membrane of giant unilamellar vesicles.Biophysical journal · 2009Article
- How cofilin severs an actin filament.Biophysical reviews · 2009Article
- Spatial Segregation of Phosphatidylinositol 4,5-Bisphosphate (PIP(2)) Signaling in Immune Cell Functions.Immunology, endocrine & metabolic agents in medicinal chemistry · 2008Article
- Giant unilamellar vesicles containing phosphatidylinositol(4,5)bisphosphate: characterization and functionality.Biophysical journal · 2008Article
- Arp2/3 controls the motile behavior of N-WASP-functionalized GUVs and modulates N-WASP surface distribution by mediating transient links with actin filaments.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vesicular trafficking and actin dynamics on Golgi membranes are both regulated by ADP-ribosylation factor 1 (ARF1) through the recruitment of various effectors, including vesicular coats. Actin assembly on Golgi membranes contributes to the architecture of the Golgi complex, vesicle formation, and trafficking and is mediated by ARF1 through a cascade that leads to Arp2/3 complex activation. Here we addressed the role of Golgi actin downstream of ARF1 by using a biomimetic assay consisting of liposomes of defined lipid composition, carrying an activated form of ARF1 incubated in cytosolic cell extracts. We observed actin polymerization around the liposomes resulting in thick actin shells and actin comet tails that pushed the ARF1 liposomes forward. The assay was used to characterize the ARF1-dependent pathway, leading to actin polymerization, and confirmed a dependency on CDC42 and its downstream effector N-WASP. Overall, this study demonstrates that actin polymerization driven by the complex multicomponent signaling cascade of the Golgi apparatus can be reproduced with a biomimetic system. Moreover, our results are consistent with the view that actin-based force generation at the site of vesicle formation contributes to the mechanism of fission. In addition to its well established function in coat recruitment, the ARF1 machinery also might produce movement- and fission-promoting forces through actin polymerization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.