ArticleNucleic acids research2007
Characterization of bacterial operons consisting of two tubulins and a kinesin-like gene by the novel Two-Step Gene Walking method.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 82 citations in OpenAlex.
- Multidomain ribosomal protein trees and the planctobacterial origin of neomura (eukaryotes, archaebacteria).Protoplasma · 2020Review
- Genome-based analysis for the identification of genes involved in o-xylene degradation in Rhodococcus opacus R7.BMC genomics · 2018Article
- Bacterial Tubulins A and B Exhibit Polarized Growth, Mixed-Polarity Bundling, and Destabilization by GTP Hydrolysis.Journal of bacteriology · 2017Article
- Four-stranded mini microtubules formed byProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Bacterial kinesin light chain (Bklc) links the Btub cytoskeleton to membranes.Scientific reports · 2017Article
- Biodegradation of the organic disulfide 4,4'-dithiodibutyric acid by Rhodococcus spp.Applied and environmental microbiology · 2015Article
- Contact- and Protein Transfer-Dependent Stimulation of Assembly of the Gliding Motility Machinery in Myxococcus xanthus.PLoS genetics · 2015Article
- Detection and genomic characterization of motility in Lactobacillus curvatus: confirmation of motility in a species outside the Lactobacillus salivarius clade.Applied and environmental microbiology · 2015Article
- Article
- Waste water effluent contributes to the dissemination of CTX-M-15 in the natural environment.The Journal of antimicrobial chemotherapy · 2014Article
- A simple one-step PCR walking method and its application of bacterial rRNA for sequencing identification.Current microbiology · 2014Article
- A simple one-step PCR walking method and its application of bacterial rRNA for sequencing identification.Current microbiology · 2014Article
- Functional screening of antibiotic resistance genes from a representative metagenomic library of food fermenting microbiota.BioMed research international · 2014Article
- The genome of the Lactobacillus sanfranciscensis temperate phage EV3.BMC research notes · 2013Article
- Succinyl-CoA:3-sulfinopropionate CoA-transferase from Variovorax paradoxus strain TBEA6, a novel member of the class III coenzyme A (CoA)-transferase family.Journal of bacteriology · 2013Article
- A novel 3-sulfinopropionyl coenzyme A (3SP-CoA) desulfinase from Advenella mimigardefordensis strain DPN7T acting as a key enzyme during catabolism of 3,3'-dithiodipropionic acid is a member of the acyl-CoA dehydrogenase superfamily.Journal of bacteriology · 2013Article
- The bacterial cytoskeleton: more than twisted filaments.Current opinion in cell biology · 2013Review
- Molecular characterization of a novel mosaic tet(S/M) gene encoding tetracycline resistance in foodborne strains of Streptococcus bovis.Microbiology (Reading, England) · 2012Article
- Complete genome sequence of the African dairy isolate Streptococcus infantarius subsp. infantarius strain CJ18.Journal of bacteriology · 2012Article
- Acinetobacter insertion sequence ISAba11 belongs to a novel family that encodes transposases with a signature HHEK motif.Applied and environmental microbiology · 2012Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tubulins are still considered as typical proteins of Eukaryotes. However, more recently they have been found in the unusual bacteria Prosthecobacter (btubAB). In this study, the genomic organization of the btub-genes and their genomic environment were characterized by using the newly developed Two-Step Gene Walking method. In all investigated Prosthecobacters, btubAB are organized in a typical bacterial operon. Strikingly, all btub-operons comprise a third gene with similarities to kinesin light chain sequences. The genomic environments of the characterized btub-operons are always different. This supports the hypothesis that this group of genes represents an independent functional unit, which was acquired by Prosthecobacter via horizontal gene transfer. The newly developed Two-Step Gene Walking method is based on randomly primed polymerase chain reaction (PCR). It presents a simple workflow, which comprises only two major steps--a Walking-PCR with a single specific outward pointing primer (step 1) and the direct sequencing of its product using a nested specific primer (step 2). Two-Step Gene Walking proved to be highly efficient and was successfully used to characterize over 20 kb of sequence not only in pure culture but even in complex non-pure culture samples.
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