ArticleThe Journal of infectious diseases2007
Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories.
Article in The Journal of infectious diseases, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 68 citations in OpenAlex.
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- IQGAP1 and IQGAP3 are critical host factors for Marburg virus replication, nucleocapsid transport, and cell-to-cell spread.Cellular and molecular life sciences : CMLS · 2026Article
- A high-throughput, polymerase-targeted RT-PCR for broad detection of mammalian filoviruses.Microbiology spectrum · 2024Article
- Sequence optimized diagnostic assay for Ebola virus detection.Scientific reports · 2023Article
- Development of a Pan-Viruses · 2023Article
- Diagnostics of Ebola virus.Frontiers in public health · 2023Review
- Reduced IFN-ß inhibitory activity of Lagos bat virus phosphoproteins in human compared to Eidolon helvum bat cells.PloS one · 2022Article
- Serological Evidence for Henipa-like and Filo-like Viruses in Trinidad Bats.The Journal of infectious diseases · 2020Article
- Development and Evaluation of a DuoMicroorganisms · 2019Article
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- The utility and perspectives of NGS-based methods in BSL-3 and BSL-4 laboratory - sequencing and analysis strategies.Briefings in functional genomics · 2018Review
- Status, quality and specific needs of Ebola virus diagnostic capacity and capability in laboratories of the two European preparedness laboratory networks EMERGE and EVD-LabNet.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2018Article
- Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future.Virology journal · 2018Article
- Field-deployable, quantitative, rapid identification of active Ebola virus infection in unprocessed blood.Chemical science · 2017Article
- Article
- South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory.PLoS neglected tropical diseases · 2017Article
- External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa.PLoS neglected tropical diseases · 2017Article
- An Outbreak of Ebola Virus Disease in the Lassa Fever Zone.The Journal of infectious diseases · 2016Article
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.