ArticleRNA (New York, N.Y.)2007
Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Enzymology of the metazoan tRNA ligase complex: a lifetime in cycles.Cellular and molecular life sciences : CMLS · 2026Review
- Intron Retention and Alzheimer's Disease (AD): A Review of Regulation Genes Implicated in AD.Genes · 2025Review
- The ChloroplastCells · 2021Review
- U5 snRNA Interactions With Exons Ensure Splicing Precision.Frontiers in genetics · 2021Article
- Role of the central junction in folding topology of the protein-free human U2-U6 snRNA complex.RNA (New York, N.Y.) · 2020Article
- Comparison of Alternative Splicing Junction Detection Tools Using RNA-Seq Data.Current genomics · 2017Review
- Group II intron lariat: Structural insights into the spliceosome.RNA biology · 2015Review
- Origin of spliceosomal introns and alternative splicing.Cold Spring Harbor perspectives in biology · 2014Article
- A day in the life of the spliceosome.Nature reviews. Molecular cell biology · 2014Review
- Conformational heterogeneity of the protein-free human spliceosomal U2-U6 snRNA complex.RNA (New York, N.Y.) · 2013Article
- Structure of the yeast U2/U6 snRNA complex.RNA (New York, N.Y.) · 2012Article
- Spliceosome structure and function.Cold Spring Harbor perspectives in biology · 2011Review
- Metal binding and substrate positioning by evolutionarily invariant U6 sequences in catalytically active protein-free snRNAs.RNA (New York, N.Y.) · 2010Article
- The tertiary structure of group II introns: implications for biological function and evolution.Critical reviews in biochemistry and molecular biology · 2010Review
- Model systems: how chemical biologists study RNA.Current opinion in chemical biology · 2009Review
- The spliceosome as ribozyme hypothesis takes a second step.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Protein-free small nuclear RNAs catalyze a two-step splicing reaction.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps.RNA (New York, N.Y.) · 2009Article
- Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.Molecular bioSystems · 2009Review
- Identification and characterization of a short 2'-3' bond-forming ribozyme.RNA (New York, N.Y.) · 2009Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Splicing of introns from mRNA precursors is a two-step reaction performed by the spliceosome, an immense cellular machine consisting of over 200 different proteins and five small RNAs (snRNAs). We previously demonstrated that fragments of two of these RNAs, U6 and U2, can catalyze by themselves a splicing-related reaction, involving one of the two substrates of the first step of splicing, the branch site substrate. Here we show that these same RNAs can catalyze a reaction between RNA sequences that resemble the 5' splice site and the branch site, the two reactants of the first step of splicing. The reaction is dependent on the sequence of the 5' splice site consensus sequence and the catalytically essential domains of U6, and thus it resembles the authentic splicing reaction. Our results demonstrate the ability of protein-free snRNAs to recognize the sequences involved in the first splicing step and to perform splicing-related catalysis between these two pre-mRNA-like substrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.