ArticleThe Journal of cell biology2007
Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- SP1 antagonizes H3K27me3 to shape chromatin landscapes for RNA polymerase II recruitment during gastrulation.Nucleic acids research · 2026Article
- DEAD-box ATPase Dbp2 is the key enzyme in an mRNP assembly checkpoint at the 3'-end of genes and involved in the recycling of cleavage factors.Nature communications · 2024Article
- Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.Nature reviews. Molecular cell biology · 2017Review
- Introns and gene expression: cellular constraints, transcriptional regulation, and evolutionary consequences.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.Development (Cambridge, England) · 2014Article
- Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation.The EMBO journal · 2012Article
- Requirement of TFIIH kinase subunit Mat1 for RNA Pol II C-terminal domain Ser5 phosphorylation, transcription and mRNA turnover.Nucleic acids research · 2011Article
- Functional cooperation between the proteins Nck and ADAP is fundamental for actin reorganization.Molecular and cellular biology · 2011Article
- Genetic organization, length conservation, and evolution of RNA polymerase II carboxyl-terminal domain.Molecular biology and evolution · 2010Article
- Article
- Dynamic changes in the human methylome during differentiation.Genome research · 2010Article
- Poly(A) signal-dependent degradation of unprocessed nascent transcripts accompanies poly(A) signal-dependent transcriptional pausing in vitro.RNA (New York, N.Y.) · 2010Article
- Nuclear networking fashions pre-messenger RNA and primary microRNA transcripts for function.Trends in cell biology · 2010Review
- "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions.Molecular cell · 2009Review
- Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.RNA (New York, N.Y.) · 2009Article
- The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation.Genes & development · 2008Article
- Quality control of mRNP in the nucleus.Chromosoma · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-mRNAs and prevents their flow to the cytoplasm by tethering them near the site of transcription. Here we provide evidence that mRNA release from the transcription site requires the heptad repeat structure of the C-terminal domain (CTD) of RNA polymerase II. The mammalian CTD, which is essential for normal co-transcriptional maturation of mRNA precursors, comprises 52 heptad repeats. We show that a truncated CTD containing 31 repeats (heptads 1-23, 36-38, and 48-52) is sufficient to support transcription, splicing, cleavage, and polyadenylation. Yet, the resulting mRNAs are mostly retained in the vicinity of the gene after transcriptional shutoff. The retained mRNAs maintain the ability to recruit components of the exon junction complex and the nuclear exosome subunit Rrp6p, suggesting that binding of these proteins is not sufficient for RNA release. We propose that the missing heptads in the truncated CTD mutant are required for binding of proteins implicated in a final co-transcriptional maturation of spliced and 3' end cleaved and polyadenylated mRNAs into export-competent ribonucleoprotein particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.