ArticleInfection and immunity2008
Essential role of chitinase in the development of the filarial nematode Acanthocheilonema viteae.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Molecular identification and functional analysis of chitinase genes reveal their importance in the metamorphosis of Sarcophaga peregrina (Diptera: Sarcophagidae).Journal of insect science (Online) · 2023Article
- Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition.Parasites & vectors · 2023Article
- Molecular cloning, heterologous expression, and in silico sequence analysis of Enterobacter GH19 class I chitinase (chiRAM gene).Molecular biology reports · 2022Article
- Discovery of novel inhibitors targeting nematode chitinase CFrontiers in chemistry · 2022Article
- Structure-based virtual screening of highly potent inhibitors of the nematode chitinaseJournal of enzyme inhibition and medicinal chemistry · 2021Article
- A Helminth-Derived Chitinase Structurally Similar to Mammalian Chitinase Displays Immunomodulatory Properties in Inflammatory Lung Disease.Journal of immunology research · 2021Article
- Chitotriosidase: a marker and modulator of lung disease.European respiratory review : an official journal of the European Respiratory Society · 2020Review
- Identification of candidate infection genes from the model entomopathogenic nematode Heterorhabditis bacteriophora.BMC genomics · 2017Article
- Does 24bp Duplication of HumanJournal of clinical and diagnostic research : JCDR · 2016Article
- Genome wide comprehensive analysis and web resource development on cell wall degrading enzymes from phyto-parasitic nematodes.BMC plant biology · 2015Article
- Article
- Dual silencing of long and short Amblyomma americanum acidic chitinase forms weakens the tick cement cone stability.The Journal of experimental biology · 2014Article
- Dual protonophore-chitinase inhibitors dramatically affect O. volvulus molting.Journal of medicinal chemistry · 2014Article
- Proteomic analysis of the excretory-secretory products from larval stages of Ascaris suum reveals high abundance of glycosyl hydrolases.PLoS neglected tropical diseases · 2013Article
- Nucleic acid transfection and transgenesis in parasitic nematodes.Parasitology · 2012Review
- Efficient in vitro RNA interference and immunofluorescence-based phenotype analysis in a human parasitic nematode, Brugia malayi.Parasites & vectors · 2012Article
- Gene expression rhythms in the mussel Mytilus galloprovincialis (Lam.) across an annual cycle.PloS one · 2011Article
- Analyses of an expressed sequence tag library from Taenia solium, Cysticerca.PLoS neglected tropical diseases · 2010Article
- Repositioning of an existing drug for the neglected tropical disease Onchocerciasis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Solid-phase synthesis of cyclic peptide chitinase inhibitors: SAR of the argifin scaffold.Organic & biomolecular chemistry · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitinases of pathogens have been proposed as potential targets of vaccines or specific inhibitors. We studied the genomic organization, transcript levels, developmental expression, and biological function of chitinases in the rodent filarial nematode Acanthocheilonema viteae, a model organism for human-pathogenic filarial worms. Characterization of nine genomic clones from an A. viteae phage library and Southern blot experiments revealed the existence of three different chitinase genes, two of which could theoretically yield functional transcripts. The deduced proteins of these genes had the common modular organization of family 18 chitinases. Northern blot experiments and rapid amplification of cDNA ends-PCR with adult worms and larval stages showed that only one gene is expressed, with high variation in transcript levels, as determined by real-time PCR. Chitinase transcript levels were lowest in the late male stage 4 larva (L4) and peaked in the stage 3 larva (L3), which was corroborated by Western blotting. RNA interference (RNAi) experiments showed that treatment of L3 and adult female worms with double-stranded RNA of chitinase inhibited molting of L3 worms and hatching of microfilariae. RNAi also led to the death of 50% of female worms, revealing the essential role of chitinase in the life cycle of filarial nematodes.
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