ArticleJournal of neuroscience methods2008
Microdialysis methods for in vivo neuropeptide measurement in the stalk-median eminence in the Rhesus monkey.
Article in Journal of neuroscience methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Trial
- Estrogen receptor-α signaling in tanycytes lies at the crossroads of fertility and metabolism.Metabolism: clinical and experimental · 2024Article
- Current Approaches to Monitor Macromolecules Directly from the Cerebral Interstitial Fluid.Pharmaceutics · 2022Review
- Mechanism of pulsatile GnRH release in primates: Unresolved questions.Molecular and cellular endocrinology · 2019Review
- Role of Kisspeptin and NKB in Puberty in Nonhuman Primates: Sex Differences.Seminars in reproductive medicine · 2019Review
- Role of Kisspeptin and Neurokinin B Signaling in Male Rhesus Monkey Puberty.Endocrinology · 2018Article
- Neuroestradiol in regulation of GnRH release.Hormones and behavior · 2018Review
- Obligatory role of hypothalamic neuroestradiol during the estrogen-induced LH surge in female ovariectomized rhesus monkeys.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Kisspeptin and Neurokinin B Signaling Network Underlies the Pubertal Increase in GnRH Release in Female Rhesus Monkeys.Endocrinology · 2017Article
- Reducing adsorption to improve recovery and in vivo detection of neuropeptides by microdialysis with LC-MS.Analytical chemistry · 2015Article
- Mass spectrometric analysis of spatio-temporal dynamics of crustacean neuropeptides.Biochimica et biophysica acta · 2015Review
- Acute Influences of Bisphenol A Exposure on Hypothalamic Release of Gonadotropin-Releasing Hormone and Kisspeptin in Female Rhesus Monkeys.Endocrinology · 2015Article
- Prolonged infusion of estradiol benzoate into the stalk median eminence stimulates release of GnRH and kisspeptin in ovariectomized female rhesus macaques.Endocrinology · 2015Article
- Neuroestradiol in the hypothalamus contributes to the regulation of gonadotropin releasing hormone release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Kisspeptin and puberty in mammals.Advances in experimental medicine and biology · 2013Review
- Developmental increase in kisspeptin-54 release in vivo is independent of the pubertal increase in estradiol in female rhesus monkeys (Macaca mulatta).Endocrinology · 2012Article
- Developmental changes in GnRH release in response to kisspeptin agonist and antagonist in female rhesus monkeys (Macaca mulatta): implication for the mechanism of puberty.Endocrinology · 2012Article
- Discovering new bioactive neuropeptides in the striatum secretome using in vivo microdialysis and versatile proteomics.Molecular & cellular proteomics : MCP · 2009Article
- Discovery of potent kisspeptin antagonists delineate physiological mechanisms of gonadotropin regulation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Direct measurement of neuropeptides in the hypothalamus is essential for neuroendocrine studies. However, the small quantities of peptides released at their neuroterminals and relatively large molecular sizes make these measurements difficult. We have evaluated microdialysis probes with two membrane materials (polycarbonate and polyarylethersulfone, both: molecular cut off 20,000 Da) in vitro, and adapted the method for in vivo hypothalamic sample collection in non-human primates. The results of in vitro experiments showed that the polyarylethersulfone membrane yielded a several fold higher recovery rate than the polycarbonate membrane. In in vivo experiments, a guide cannula with stylet was inserted into the medial basal hypothalamus through the permanently implanted cranial pedestal under light sedation. The stylet was replaced by a microdialysis probe and artificial CSF was infused. The results indicated that the neuropeptide luteinizing hormone-releasing hormone was readily measurable in dialysates collected at 10 min-intervals, and responded to neuroactive substances applied through the probe. The animals were fully conscious except for the initial hour of sampling. After the experiment the animal was returned to the home cage, and later similarly examined during several additional experiments. Therefore, the microdialysis method described here is a highly useful tool for neuroendocrine studies in non-human primates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.