ArticleBone2007
Calcospherulites isolated from the mineralization front of bone induce the mineralization of type I collagen.
Article in Bone, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Healing Kinetics of Sinus Lift Augmentation Using Biphasic Calcium Phosphate Granules: A Case Series in Humans.Bioengineering (Basel, Switzerland) · 2025Article
- Review
- Zinc-alkaline phosphatase at sites of aortic calcification.Journal of molecular histology · 2024Article
- Mesoscale characterization of osseointegration around an additively manufactured genistein-coated implant.Scientific reports · 2024Article
- Bone without borders - Monetite-based calcium phosphate guides bone formation beyond the skeletal envelope.Bioactive materials · 2023Article
- Advances in biomimetic collagen mineralisation and future approaches to bone tissue engineering.Biopolymers · 2023Review
- Re-Evaluation of Initial Bone Mineralization from an Engineering Perspective.Tissue engineering. Part B, Reviews · 2022Review
- Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration.Science and technology of advanced materials · 2021Article
- Transformation of bone mineral morphology: From discrete marquise-shaped motifs to a continuous interwoven mesh.Bone reports · 2020Article
- Review
- Article
- Article
- Mast Cell Mediators Inhibit Osteoblastic Differentiation and Extracellular Matrix Mineralization.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX spectroscopy, XRD, and FTIR spectroscopy.Journal of bone and mineral metabolism · 2016Article
- Biologic Potential of Calcium Phosphate Biopowders Produced via Decomposition Combustion Synthesis.Ceramics international · 2015Article
- Osteocyte regulation of bone mineral: a little give and take.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2012Review
- Article
- Biomineralization of bone: a fresh view of the roles of non-collagenous proteins.Frontiers in bioscience (Landmark edition) · 2011Review
- Dentin: structure, composition and mineralization.Frontiers in bioscience (Elite edition) · 2011Review
- Nanomechanical characterization of tissue engineered bone grown on titanium alloy in vitro.Journal of materials science. Materials in medicine · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Previous work has suggested that "calcospherulites" actively participate in the mineralization of developing and healing bone. This study sought to directly test this hypothesis by developing a method to isolate calcospherulites and analyzing their capacity to seed mineralization of fibrillar collagen. The periosteal surface of juvenile rat tibial diaphysis was enriched in spherulites of approximately 0.5-mum diameter exhibiting a Ca/P ratio of 1.3. Their identity as calcospherulites was confirmed by their uptake of calcein at the tibial mineralization front 24 h following in vivo injection. Periosteum was dissected and unmineralized osteoid removed by collagenase in order to expose calcospherulites. Calcein-labeled calcospherulites were then released from the mineralization front by dispase digestion and isolated via fluorescence flow sorting. X-ray diffraction analysis revealed they contained apatite crystals (c-axis length of 17.5+/-0.2 nm), though their Ca/P ratio of 1.3 is lower than that of hydroxyapatite. Much of their non-mineral phosphorous content was removed by ice-cold ethanol, elevating their Ca/P ratio to 1.6, suggesting the presence of phospholipids. Western blot analyses showed the presence of bone matrix proteins and type I collagen in these preparations. Incubating isolated calcospherulites in collagen hydrogels demonstrated that they could seed a mineralization reaction on type I collagen fibers in vitro. Ultrastructural analyses revealed crystals on the collagen fibers that were distributed rather uniformly along the fiber lengths. Furthermore, crystals were observed at distances well away from the observed calcospherulites. Our results directly support an active role for calcospherulites in inducing the mineralization of type I collagen fibers at the mineralization front of bone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.