ArticleApplied and environmental microbiology2007
Phenotypic and transcriptomic analyses demonstrate interactions between the transcriptional regulators CtsR and Sigma B in Listeria monocytogenes.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.World journal of microbiology & biotechnology · 2025Review
- Protein Interaction Network Analysis to Investigate Stress Response, Virulence, and Antibiotic Resistance Mechanisms inMicroorganisms · 2023Article
- Roles of Alternative Sigma Factors in Invasion and Growth Characteristics ofFrontiers in microbiology · 2022Article
- Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments.Scientific reports · 2021Article
- Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L inMicroorganisms · 2020Article
- Cross Talk between SigB and PrfA in Listeria monocytogenes Facilitates Transitions between Extra- and Intracellular Environments.Microbiology and molecular biology reviews : MMBR · 2019Review
- Exploring Listeria monocytogenes Transcriptomes in Correlation with Divergence of Lineages and Virulence as Measured in Galleria mellonella.Applied and environmental microbiology · 2019Article
- Establishment ofMicroorganisms · 2019Review
- Inactivation of Heat Adapted and Chlorine AdaptedItalian journal of food safety · 2017Article
- Transcriptomic Analysis of the Adaptation ofFrontiers in microbiology · 2017Article
- Article
- Listeria monocytogenes DNA Glycosylase AdlP Affects Flagellar Motility, Biofilm Formation, Virulence, and Stress Responses.Applied and environmental microbiology · 2016Article
- Transcriptional and phenotypic responses of Listeria monocytogenes to chlorine dioxide.Applied and environmental microbiology · 2014Article
- Regulatory network features in Listeria monocytogenes-changing the way we talk.Frontiers in cellular and infection microbiology · 2014Review
- Article
- Gene expression profiling of a nisin-sensitive Listeria monocytogenes Scott A ctsR deletion mutant.Journal of industrial microbiology & biotechnology · 2013Article
- Article
- Divergence of the SigB regulon and pathogenesis of the Bacillus cereus sensu lato group.BMC genomics · 2012Article
- Listeria monocytogenes grown at 7° C shows reduced acid survival and an altered transcriptional response to acid shock compared to L. monocytogenes grown at 37° C.Applied and environmental microbiology · 2012Article
- Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes sigma(B) positively regulates the transcription of class II stress response genes; CtsR negatively regulates class III stress response genes. To identify interactions between these two stress response systems, we constructed L. monocytogenes DeltactsR and DeltactsR DeltasigB strains, as well as a DeltactsR strain expressing ctsR in trans under the control of an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter. These strains, along with a parent and a DeltasigB strain, were assayed for motility, heat resistance, and invasion of human intestinal epithelial cells, as well as by whole-genome transcriptomic and quantitative real-time PCR analyses. Both DeltactsR and DeltactsR DeltasigB strains had significantly higher thermotolerances than the parent strain; however, full heat sensitivity was restored to the DeltactsR strain when ctsR was expressed in trans. Although log-phase DeltactsR was not reduced in its ability to infect human intestinal cells, the DeltactsR DeltasigB strain showed significantly lower invasion efficiency than either the parent strain or the DeltasigB strain, indicating that interactions between CtsR and sigma(B) contribute to invasiveness. Statistical analyses also confirmed interactions between the ctsR and the sigB null mutations in both heat resistance and invasion phenotypes. Microarray transcriptomic analyses and promoter searches identified (i) 42 CtsR-repressed genes, (ii) 22 genes with lower transcript levels in the DeltactsR strain, and (iii) at least 40 genes coregulated by both CtsR and sigma(B), including genes encoding proteins with confirmed or plausible roles in virulence and stress response. Our data demonstrate that interactions between CtsR and sigma(B) play an important role in L. monocytogenes stress resistance and virulence.
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