ArticleApplied and environmental microbiology2007
Aged HCT-8 cell monolayers support Cryptosporidium parvum infection.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Apical-out bovine intestinal organoids as an infection model forCurrent research in parasitology & vector-borne diseases · 2025Article
- Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.PLoS neglected tropical diseases · 2021Article
- Intestinal organoid/enteroid-based models for Cryptosporidium.Current opinion in microbiology · 2020Review
- Article
- Past and future trends of Cryptosporidium in vitro research.Experimental parasitology · 2019Review
- Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept.Scientific reports · 2017Article
- Genome-wide upstream motif analysis of Cryptosporidium parvum genes clustered by expression profile.BMC genomics · 2013Article
- Prolonged NF-κB activation by a macrophage inhibitory cytokine 1-linked signal in enteropathogenic Escherichia coli-infected epithelial cells.Infection and immunity · 2013Article
- Enhanced wound healing by recombinant Escherichia coli Nissle 1917 via human epidermal growth factor receptor in human intestinal epithelial cells: therapeutic implication using recombinant probiotics.Infection and immunity · 2012Article
- Comparison of assays for sensitive and reproducible detection of cell culture-infectious Cryptosporidium parvum and Cryptosporidium hominis in drinking water.Applied and environmental microbiology · 2012Article
- Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma.The Journal of biological chemistry · 2010Article
- Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells.Molecular and biochemical parasitology · 2009Article
- Revisiting the global problem of cryptosporidiosis and recommendations.Tropical parasitologyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell culture assays in various formats have been used to study the infectivity of Cryptosporidium spp. as well as to determine the infectivity of naturally occurring oocysts in water. Currently, cell culture assays for infectious Cryptosporidium spp. in water have largely been limited to practice in research laboratories. One obstacle to the routine use of Cryptosporidium cell culture assays for the analysis of water samples is the coordination of water sample collection and processing with readiness of cell culture monolayers. For most Cryptosporidium cell culture assays, monolayers are allowed to develop for 24 to 48 h to reach 80 to 100% confluence prior to inoculation. In this study, we used immunofluorescent assay microscopy to evaluate freshly confluent (2-day-old) and aged (8- to 67-day-old) HCT-8 cell monolayers for their ability to support Cryptosporidium parvum infection. HCT-8 monolayers as old as 67 days were clearly shown to support infection. In two of three experiments, aged monolayers (8- to 11-day-old and 11- to 22-day-old, respectively) developed the same number of C. parvum clusters of infection as freshly confluent monolayers. Results suggest that it may be possible to use cell monolayers from freshly confluent to 3 weeks old on hand for infectivity assays without having to schedule sample processing to coincide with development of freshly confluent monolayers. This would make Cryptosporidium cell culture assays much more feasible for water quality and utility laboratories.
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