ArticleThe Plant cell2007
Subcellular localization and functional domain studies of DEFECTIVE KERNEL1 in maize and Arabidopsis suggest a model for aleurone cell fate specification involving CRINKLY4 and SUPERNUMERARY ALEURONE LAYER1.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of the expression profiles of the Arabidopsis ESCRT machinery.Plant signaling & behavior · 2011Pooled it
- Calpains as Potential Regulators of Microtubule-Associated Cytokinesis: A Cross-Eukaryotic Unifying Hypothesis.Life (Basel, Switzerland) · 2026Article
- The aleurone layer of cereal grains: Development, genetic regulation, and breeding applications.Plant communications · 2025Review
- CLE peptides act via the receptor-like kinase CRINKLY 4 inPlant signaling & behavior · 2024Article
- Regulation of developmental gatekeeping and cell fate transition by the calpain protease DEK1 in Physcomitrium patens.Communications biology · 2024Article
- Emerging Roles of Receptor-like Protein Kinases in Plant Response to Abiotic Stresses.International journal of molecular sciences · 2023Review
- Membrane-anchored calpains - hidden regulators of growth and development beyond plants?Frontiers in plant science · 2023Article
- The genome and preliminary single-nuclei transcriptome of Lemna minuta reveals mechanisms of invasiveness.Plant physiology · 2022Article
- Maize Endosperm Development: Tissues, Cells, Molecular Regulation and Grain Quality Improvement.Frontiers in plant science · 2022Review
- CRISPR-Cas9 mediated mutation in GRAIN WIDTH and WEIGHT2 (GW2) locus improves aleurone layer and grain nutritional quality in rice.Scientific reports · 2021Article
- Comparative transcriptomics and network analysis define gene coexpression modules that control maize aleurone development and auxin signaling.The plant genome · 2021Article
- shrunken4 is a mutant allele of ZmYSL2 that affects aleurone development and starch synthesis in maize.Genetics · 2021Article
- Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s.Scientific reports · 2020Article
- MINI SEED 2 (MIS2) Encodes a Receptor-like Kinase that Controls Grain Size and Shape in Rice.Rice (New York, N.Y.) · 2020Article
- The Beginning of the End: Initial Steps in the Degradation of Plasma Membrane Proteins.Frontiers in plant science · 2020Review
- Mapping of QTL for kernel abortion caused by in vivo haploid induction in maize (Zea mays L.).PloS one · 2020Article
- AtPR5K2, a PR5-Like Receptor Kinase, Modulates Plant Responses to Drought Stress by Phosphorylating Protein Phosphatase 2Cs.Frontiers in plant science · 2019Article
- Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.).Scientific reports · 2018Article
- zmsbt1 and zmsbt2, two new subtilisin-like serine proteases genes expressed in early maize kernel development.Planta · 2017Article
- Extra Large G-Protein Interactome Reveals Multiple Stress Response Function and Partner-Dependent XLG Subcellular Localization.Frontiers in plant science · 2017Article
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11 authors.
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Abstract
DEFECTIVE KERNEL1 (DEK1), which consists of a membrane-spanning region (DEK1-MEM) and a calpain-like Cys proteinase region (DEK1-CALP), is essential for aleurone cell formation at the surface of maize (Zea mays) endosperm. Immunolocalization and FM4-64 dye incubation experiments showed that DEK1 and CRINKLY4 (CR4), a receptor kinase implicated in aleurone cell fate specification, colocalized to plasma membrane and endosomes. SUPERNUMERARY ALEURONE LAYER1 (SAL1), a negative regulator of aleurone cell fate encoding a class E vacuolar sorting protein, colocalized with DEK1 and CR4 in endosomes. Immunogold localization, dual-axis electron tomography, and diffusion of fluorescent dye tracers showed that young aleurone cells established symplastic subdomains through plasmodesmata of larger dimensions than those connecting starchy endosperm cells and that CR4 preferentially associated with plasmodesmata between aleurone cells. Genetic complementation experiments showed that DEK1-CALP failed to restore wild-type phenotypes in maize and Arabidopsis thaliana dek1 mutants, and DEK1-MEM also failed to restore wild-type phenotypes in Arabidopsis dek1-1 mutants. Instead, ectopic expression of DEK1-MEM under the control of the cauliflower mosaic virus 35S promoter gave a dominant negative phenotype. These data suggest a model for aleurone cell fate specification in which DEK1 perceives and/or transmits a positional signal, CR4 promotes the lateral movement of aleurone signaling molecules between aleurone cells, and SAL1 maintains the proper plasma membrane concentration of DEK1 and CR4 proteins via endosome-mediated recycling/degradation.
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