ArticleBiophysical journal2008
Sequence-dependent variations associated with H2A/H2B depletion of nucleosomes.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Nucleosome assembly and disassembly pathways in vitro.PloS one · 2022Article
- Jörg Langowski: his scientific legacy and the future it promises.BMC biophysics · 2018Article
- Nucleosome Core Particle Disassembly and Assembly Kinetics Studied Using Single-Molecule Fluorescence.Biophysical journal · 2015Article
- Opposing roles of H3- and H4-acetylation in the regulation of nucleosome structure––a FRET study.Nucleic acids research · 2015Article
- Solution scattering and FRET studies on nucleosomes reveal DNA unwrapping effects of H3 and H4 tail removal.PloS one · 2013Article
- The effect of DNA CpG methylation on the dynamic conformation of a nucleosome.Biophysical journal · 2012Article
- Nucleosome accessibility governed by the dimer/tetramer interface.Nucleic acids research · 2011Article
- Nucleosomes structure and dynamics: effect of CHAPS.International journal of biochemistry and molecular biology · 2011Article
- Long-range effects of histone point mutations on DNA remodeling revealed from computational analyses of SIN-mutant nucleosome structures.Nucleic acids research · 2010Article
- Probing biomolecular structures and dynamics of single molecules using in-gel alternating-laser excitation.Analytical chemistry · 2009Article
- Nucleosome disassembly intermediates characterized by single-molecule FRET.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Dynamics of nucleosomes revealed by time-lapse atomic force microscopy.Biochemistry · 2009Article
- spFRET using alternating excitation and FCS reveals progressive DNA unwrapping in nucleosomes.Biophysical journal · 2009Article
- DNA sequence mediates nucleosome structure and stability.Biophysical journal · 2008Article
- Expedient placement of two fluorescent dyes for investigating dynamic DNA protein interactions in real time.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Review
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7 authors at 1 institution in 1 country.
Funding
Abstract
Mechanisms that can alter nucleosome structure to enhance DNA accessibility are of great interest because of their potential involvement in genomic processes. One such mechanism is H2A/H2B release from nucleosomes; it occurs in vivo and is involved in the in vitro activities of several transcription-associated complexes. Using fluorescence approaches based on Förster resonance energy transfer, we previously detected sequence-dependent structure/stability variations between 5S and two types of promoter nucleosomes (from yeast GAL10 or mouse mammary tumor virus promoters). Those variations included differing responses when nucleosomes were diluted to concentrations (sub-nM) known to produce H2A/H2B loss. Here, we show that treatment of these same three types of nucleosomes with the histone chaperone yNAP-1, which causes H2A/H2B release from nucleosomes in vitro, produces the same differential Förster resonance energy transfer responses, again demonstrating sequence-dependent variations associated with conditions that produce H2A/H2B loss. Single-molecule population data indicate that DNA dynamics on the particles produced by diluting nucleosomes to sub-nM concentrations follow two-state behavior. Rate information (determined by fluorescence correlation spectroscopy) suggests that these dynamics are enhanced in MMTV-B or GAL10 compared to 5S particles. Taken together, the results indicate that H2A/H2B loss has differing effects on 5S compared to these two promoter nucleosomes and the differences reflect sequence-dependent structure/stability variations in the depleted particles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.