ArticleMolecular biology reports2008
Molecular cloning and characterization of a mannose-binding lectin gene from Pinellia cordata.
Article in Molecular biology reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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Who cites it
2 citing papers in PubMed.
- Effect of the lectin of Bauhinia variegata and its recombinant isoform on surgically induced skin wounds in a murine model.Molecules (Basel, Switzerland) · 2011Article
- cDNA cloning and functional expression of the alpha-D-galactose-binding lectin frutalin in Escherichia coli.Molecular biotechnology · 2009Article
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Authors and funding
8 authors.
Funding
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Abstract
Using RNA extracted from Pinellia cordata young leaves and primers designed according to the conserved regions of Araceae lectins, the full-length cDNA of Pinellia cordata agglutinin (PCL) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of pcl was 1,182 bp and contained a 768 bp open reading frame (ORF) encoding a lectin precursor of 256 amino acids. Through comparative analysis of pcl gene and its deduced amino acid sequence with those of other Araceae species, it was found that pcl encoded a precursor lectin with signal peptide. PCL is a mannose-binding lectin with three mannose-binding sites. Semi-quantitative RT-PCR analysis revealed that pcl is expressed in all tested tissues including leaf, stem and bulbil, but with the highest expression in bulbil. PCL protein was successfully expressed in Escherichia coli with the molecular weight expected.
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