ArticleMolecular biology reports2009
Molecular cloning and characterization of a profilin gene BnPFN from Brassica nigra that expressing in a pollen-specific manner.
Article in Molecular biology reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 6 citations in OpenAlex.
- Analysis of Pollen Allergens in Lily by Transcriptome and Proteome Data.International journal of molecular sciences · 2019Article
- Characterization of Betula platyphylla gene transcripts associated with early development of male inflorescence.Molecular biology reports · 2012Article
- Parallel up-regulation of the profilin gene family following independent domestication of diploid and allopolyploid cotton (Gossypium).Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Isolation and characterization of a novel BcMF14 gene from Brassica campestris ssp. chinensis.Molecular biology reports · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Brassica nigra is a newly found invasive species in Zhejiang Province, China. It distributes alongside the roads, in vegetable fields and on riversides. When it blooms, some natives there will suffer from allergic rhinitis. We designed gene-specific primer pairs according to reported profilin genes and successfully isolated their homolog from flower bud cDNA of B. nigra. The gene, designated BnPFN, was submitted to GenBank under accession number EU004073. BnPFN was 405 bp in length encoding 134 amino acids. Expression analysis of BnPFN gene was carried out by means of RT-PCR. The results showed that BnPFN express only in anthers and pollens, and there was no detection in roots, leaves, stems, sepals, petals and pistils. We suggest that BnPFN is a pollen-specific gene and may be responsible for pollen anaphylactic reactions in those invading areas when B. nigra blooms.
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