ArticleClinical & experimental metastasis2008
Contaminating cells alter gene signatures in whole organ versus laser capture microdissected tumors: a comparison of experimental breast cancers and their lymph node metastases.
Article in Clinical & experimental metastasis, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Prognostic relevance of molecular subtypes and master regulators in pancreatic ductal adenocarcinoma.BMC cancer · 2016Trial
- Separation of breast cancer and organ microenvironment transcriptomes in metastases.Breast cancer research : BCR · 2019Article
- Computer-Aided Laser Dissection: A Microdissection Workflow Leveraging Image Analysis Tools.Journal of pathology informatics · 2018Article
- Quantitative proteomics analysis of mitochondrial proteins in lung adenocarcinomas and normal lung tissue using iTRAQ and tandem mass spectrometry.American journal of translational research · 2017Article
- miRNA expression and function in thyroid carcinomas: a comparative and critical analysis and a model for other cancers.Oncotarget · 2016Review
- New global analysis of the microRNA transcriptome of primary tumors and lymph node metastases of papillary thyroid cancer.BMC genomics · 2015Article
- Impact of upfront cellular enrichment by laser capture microdissection on protein and phosphoprotein drug target signaling activation measurements in human lung cancer: Implications for personalized medicine.Proteomics. Clinical applications · 2015Article
- SIVQ-LCM protocol for the ArcturusXT instrument.Journal of visualized experiments : JoVE · 2014Article
- Transcriptional dissection of pancreatic tumors engrafted in mice.Genome medicine · 2014Article
- Three-dimensional mRNA measurements reveal minimal regional heterogeneity in esophageal squamous cell carcinoma.The American journal of pathology · 2013Article
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- Integrated proteomic, transcriptomic, and biological network analysis of breast carcinoma reveals molecular features of tumorigenesis and clinical relapse.Molecular & cellular proteomics : MCP · 2012Article
- EGFR and KRAS mutation analysis in cytologic samples of lung adenocarcinoma enabled by laser capture microdissection.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2012Article
- Dissection of stromal and cancer cell-derived signals in melanoma xenografts before and after treatment with DMXAA.British journal of cancer · 2012Article
- Why is it crucial to reintegrate pathology into cancer research?BioEssays : news and reviews in molecular, cellular and developmental biology · 2011Article
- SIVQ-aided laser capture microdissection: A tool for high-throughput expression profiling.Journal of pathology informatics · 2011Article
- Methylation profile of single hepatocytes derived from hepatitis B virus-related hepatocellular carcinoma.PloS one · 2011Article
- Validation of a mouse xenograft model system for gene expression analysis of human acute lymphoblastic leukaemia.BMC genomics · 2010Article
- A gene expression signature that defines breast cancer metastases.Clinical & experimental metastasis · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Genome-wide expression profiling has expedited our molecular understanding of the different subtypes of breast cancers, as well as defined the differences among genes expressed in primary tumors and their metastases. Laser-capture microdissection (LCM) coupled to gene expression analysis allows us to understand how specific cell types contribute to the total cancer gene expression signature. Expression profiling was used to define genes that contribute to breast cancer spread into and/or growth within draining lymph nodes (LN). Whole tumor xenografts and their matched whole LN metastases were compared to LCM captured cancer cells from the same tumors and matched LN metastases. One-thousand nine-hundred thirty genes were identified by the whole organ method alone, and 1,281 genes by the LCM method alone. However, less than 1% (30 genes) of genes that changed between tumors and LN metastases were common to both methods. Several of these genes have previously been implicated in cancer aggressiveness. Our data show that whole-organ and LCM based gene expression profiling yield distinctly different lists of metastasis-promoting genes. Contamination of the tumor cells, and cross reactivity of mouse RNA to human-specific chips may explain these differences, and suggests that LCM-derived data may be more accurate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.