ArticlePlant physiology2007
A ligation-independent cloning tobacco rattle virus vector for high-throughput virus-induced gene silencing identifies roles for NbMADS4-1 and -2 in floral development.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
81 citing papers in PubMed.
- Evolutionary hijacking of fatty acid biosynthesis drives insecticidal acylsugar diversity in tomato.Nature communications · 2026Article
- A viral protein diverts the endocytic VPS9a-Rab5 pathway to promote virus infection in plants.Science advances · 2026Article
- Evolutionary diversification of acyl-CoA synthetases underpins hydrophobic barrier formation across diverse tomato tissues and beyond.Horticulture research · 2025Article
- Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism.The New phytologist · 2025Article
- Genome-Wide Analysis Elucidates the Roles ofPlants (Basel, Switzerland) · 2024Article
- Multiple independent losses of the biosynthetic pathway for two tropane alkaloids in the Solanaceae family.Nature communications · 2023Article
- MEDIATOR SUBUNIT 16 negatively regulates rice immunity by modulating PATHOGENESIS RELATED 3 activity.Plant physiology · 2023Article
- Convergent evolution of immune receptors underpins distinct elicitin recognition in closely related Solanaceous plants.The Plant cell · 2023Article
- Redirecting tropane alkaloid metabolism reveals pyrrolidine alkaloid diversity in Atropa belladonna.The New phytologist · 2023Article
- Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components.Plant physiology · 2023Article
- Expression and Functional Studies of Leaf, Floral, and Fruit Developmental Genes in Non-model Species.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Natural variation meets synthetic biology: Promiscuous trichome-expressed acyltransferases from Nicotiana.Plant physiology · 2022Article
- Metabolomics-guided discovery of cytochrome P450s involved in pseudotropine-dependent biosynthesis of modified tropane alkaloids.Nature communications · 2022Article
- Characterization of two conserved cell death elicitor families from the Dothideomycete fungal pathogensFrontiers in microbiology · 2022Article
- Promoting virus-induced gene silencing of pepper genes by a heterologous viral silencing suppressor.Plant biotechnology journal · 2021Article
- Verticillium dahliae secreted protein Vd424Y is required for full virulence, targets the nucleus of plant cells, and induces cell death.Molecular plant pathology · 2021Article
- VipariNama: RNA viral vectors to rapidly elucidate the relationship between gene expression and phenotype.Plant physiology · 2021Article
- A cassava common mosaic virus vector for virus-induced gene silencing in cassava.Plant methods · 2021Article
- An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya.Horticulture research · 2021Article
- Tissue-Specific RNA-Seq Analysis and Identification of Receptor-Like Proteins Related to Plant Growth inPlants (Basel, Switzerland) · 2021Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus-induced gene silencing (VIGS) is a widely used, powerful technique for reverse genetics. VIGS vectors derived from the Tobacco rattle virus (TRV) are among the most popular for VIGS. We have developed a TRV RNA2 vector that allows the insertion of gene silencing fragments by ligation-independent cloning (LIC). This new vector has several advantages over previous vectors, particularly for applications involving the analysis of large numbers of sequences, since TRV-LIC vectors containing the desired insert are obtained with 100% efficiency. Importantly, this vector allows the high-throughput cloning of silencing fragments without the use of costly enzymes required for recombination, as is the case with GATEWAY-based vectors. We generated a collection of silencing vectors based on 400 tomato (Solanum lycopersicum) expressed sequence tags in this TRV-LIC background. We have used this vector to identify roles for SlMADS1 and its Nicotiana benthamiana homologs, NbMADS4-1 and -2 in flowering. We find that NbMADS4-1 and -2 act nonredundantly in floral development and silencing of either gene results in loss of organ identity. This TRV-LIC vector should be a valuable resource to the plant community.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.