ArticleBiotechnology and bioengineering2008
Monolayer cell expansion conditions affect the chondrogenic potential of adipose-derived stem cells.
Article in Biotechnology and bioengineering, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed.
- Modulating mesenchymal stromal cells chondrogenesis through TGF-β1 pre-conditioning during monolayer expansion in pellet culture and in a 3D mechanical load model.Journal of translational medicine · 2026Article
- Understanding molecular characteristics of extracellular vesicles derived from different types of mesenchymal stem cells for therapeutic translation.Extracellular vesicle · 2024Article
- Hydrogel Encapsulation of Genome-Engineered Stem Cells for Long-Term Self-Regulating Anti-Cytokine Therapy.Gels (Basel, Switzerland) · 2023Article
- Article
- Chondrogenesis of Adipose-Derived Stem Cells Using an Arrayed Spheroid Format.Cellular and molecular bioengineering · 2022Article
- Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Chondroprotective Effects of Combination Therapy of Acupotomy and Human Adipose Mesenchymal Stem Cells in Knee Osteoarthritis Rabbits via the GSK3β-Cyclin D1-CDK4/CDK6 Signaling Pathway.Aging and disease · 2020Article
- Breast Augmentation by Fat Transplantation With Adipose-Derived Stem/Stromal Cells.Aesthetic surgery journal. Open forum · 2020Article
- Dynamics of transcriptome changes during subcutaneous preadipocyte differentiation in ducks.BMC genomics · 2019Article
- The Effects of Different Dynamic Culture Systems on Cell Proliferation and Osteogenic Differentiation in Human Mesenchymal Stem Cells.International journal of molecular sciences · 2019Article
- Considerations for high-yield, high-throughput cell enrichment: fluorescence versus magnetic sorting.Scientific reports · 2019Article
- Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells.Biomaterials research · 2019Article
- Cell Mimicking Microparticles Influence the Organization, Growth, and Mechanophenotype of Stem Cell Spheroids.Annals of biomedical engineering · 2018Article
- Regenerative Potential and Inflammation-Induced Secretion Profile of Human Adipose-Derived Stromal Vascular Cells Are Influenced by Donor Variability and Prior Breast Cancer Diagnosis.Stem cell reviews and reports · 2018Article
- The effect of serum types on Chondrogenic differentiation of adipose-derived stem cells.Biomaterials research · 2018Article
- Processing fixed and stored adipose-derived stem cells for quantitative protein array assays.BioTechniques · 2017Article
- Environmental preconditioning rejuvenates adult stem cells' proliferation and chondrogenic potential.Biomaterials · 2017Review
- Disparate Response to Methotrexate in Stem Versus Non-Stem Cells.Stem cell reviews and reports · 2016Article
- Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering.Advanced pharmaceutical bulletin · 2016Article
- Nanomechanics of Engineered Articular Cartilage: Synergistic Influences of Transforming Growth Factor-β3 and Oscillating Pressure.Journal of nanoscience and nanotechnology · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Adipose-derived stem cells (ASCs) are an abundant, readily available population of multipotent progenitor cells that reside in adipose tissue. Isolated ASCs are typically expanded in monolayer on standard tissue culture plastic with a basal medium containing 10% fetal bovine serum. However, recent data suggest that altering the monolayer expansion conditions by using suspension culture plastic, adding growth factors to the medium, or adjusting the seeding density may affect the self-renewal rate, multipotency, and lineage-specific differentiation potential of the ASCs. We hypothesized that variation in any of these expansion conditions would influence the chondrogenic potential of ASCs. ASCs were isolated from human liposuction waste tissue and expanded through two passages with different tissue culture plastic, feed medium, and cell seeding densities. Once expanded, the cells were cast in an agarose gel and subjected to identical chondrogenic culture conditions for 7 days, at which point cell viability, radiolabel incorporation, and gene expression were measured. High rates of matrix synthesis upon chondrogenic induction were mostly associated with smaller cells, as indicated by cell width and area on tissue culture plastic, and it appears that expansion in a growth factor supplemented medium is important in maintaining this morphology. All end-point measures were highly dependent on the specific monolayer culture conditions. These results support the hypothesis that monolayer culture conditions may "prime" the cells or predispose them towards a specific phenotype and thus underscore the importance of early culture conditions in determining the growth and differentiation potential of ASCs.
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