ArticlePlant molecular biology2007
A genome-wide gain-of function analysis of rice genes using the FOX-hunting system.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- Plant Functional Genomics Based on High-Throughput CRISPR Library Knockout Screening: A Perspective.Advanced genetics (Hoboken, N.J.) · 2024Article
- Construction of a Full-Length cDNA Over-Expressing Library to Identify Valuable Genes fromInternational journal of molecular sciences · 2021Article
- Fox Hunting in Wild Apples: Searching for Novel Genes inInternational journal of molecular sciences · 2020Article
- Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation.Plant biotechnology journal · 2020Article
- High-throughput phenotypic screening of random genomic fragments in transgenic rice identified novel drought tolerance genes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics.Plants (Basel, Switzerland) · 2019Review
- Association Analysis of Three Diverse Rice (The plant genome · 2019Article
- The rice CYP78A gene BSR2 confers resistance to Rhizoctonia solani and affects seed size and growth in Arabidopsis and rice.Scientific reports · 2019Article
- Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System fromInternational journal of molecular sciences · 2018Article
- Development of iFOX-hunting as a functional genomic tool and demonstration of its use to identify early senescence-related genes in the polyploid Brassica napus.Plant biotechnology journal · 2018Article
- Ascribing Functions to Genes: Journey Towards Genetic Improvement of Rice Via Functional Genomics.Current genomics · 2016Article
- Overexpression of BSR1 confers broad-spectrum resistance against two bacterial diseases and two major fungal diseases in rice.Breeding science · 2016Article
- Rice OsVAMP714, a membrane-trafficking protein localized to the chloroplast and vacuolar membrane, is involved in resistance to rice blast disease.Plant molecular biology · 2016Article
- Gene Overexpression Resources in Cereals for Functional Genomics and Discovery of Useful Genes.Frontiers in plant science · 2016Review
- Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice.Plant biotechnology journal · 2015Article
- Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance.PloS one · 2015Article
- Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat.Protoplasma · 2014Article
- Discovery and characterization of two novel salt-tolerance genes in Puccinellia tenuiflora.International journal of molecular sciences · 2014Article
- An efficient Agrobacterium-mediated transformation system for poplar.International journal of molecular sciences · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The latest report has estimated the number of rice genes to be approximately 32,000. To elucidate the functions of a large population of rice genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the Full-length cDNA Over-eXpresser (FOX) gene-hunting system. This system is very useful for analyzing various gain-of-function phenotypes from large populations of transgenic plants overexpressing cDNAs of interest and others with unknown or important functions. We collected the plasmid DNAs of 13,980 independent full-length cDNA (FL-cDNA) clones to produce a FOX library by placing individual cDNAs under the control of the maize Ubiquitin-1 promoter. The FOX library was transformed into rice by Agrobacterium-mediated high-speed transformation. So far, we have generated approximately 12,000 FOX-rice lines. Genomic PCR analysis indicated that the average number of FL-cDNAs introduced into individual lines was 1.04. Sequencing analysis of the PCR fragments carrying FL-cDNAs from 8615 FOX-rice lines identified FL-cDNAs in 8225 lines, and a database search classified the cDNAs into 5462 independent ones. Approximately 16.6% of FOX-rice lines examined showed altered growth or morphological characteristics. Three super-dwarf mutants overexpressed a novel gibberellin 2-oxidase gene,confirming the importance of this system. We also show here the other morphological alterations caused by individual FL-cDNA expression. These dominant phenotypes should be valuable indicators for gene discovery and functional analysis.
Indexed as
Identifiers
17929174What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.