ArticleCurrent genetics2007
Detection of hyphal fusion in filamentous fungi using differently fluorescence-labeled histones.
Article in Current genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Evidence for the Existence of Mating Subtypes Within theJournal of fungi (Basel, Switzerland) · 2025Article
- STRIPAK Dependent and Independent Phosphorylation of the SIN Kinase DBF2 Controls Fruiting Body Development and Cytokinesis during Septation and Ascospore Formation inJournal of fungi (Basel, Switzerland) · 2024Article
- Construction of nucleus-directed fluorescent reporter systems and its application to verification of heterokaryon formation inFrontiers in microbiology · 2022Article
- Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.AMB Express · 2020Article
- The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes.PLoS genetics · 2020Article
- Fluorescent protein expression in the ectomycorrhizal fungus Laccaria bicolor: a plasmid toolkit for easy use of fluorescent markers in basidiomycetes.Current genetics · 2020Article
- Sordaria macrospora: 25 years as a model organism for studying the molecular mechanisms of fruiting body development.Applied microbiology and biotechnology · 2020Review
- Crosstalk Between Pheromone Signaling and NADPH Oxidase Complexes Coordinates Fungal Developmental Processes.Frontiers in microbiology · 2020Article
- Comparative Genomics and Transcriptomics To Analyze Fruiting Body Development in Filamentous Ascomycetes.Genetics · 2019Article
- A Hippo Pathway-Related GCK Controls Both Sexual and Vegetative Developmental Processes in the FungusGenetics · 2018Article
- A Xenobiotic Detoxification Pathway through Transcriptional Regulation in Filamentous Fungi.mBio · 2018Article
- BiFC-based visualisation system reveals cell fusion morphology and heterokaryon incompatibility in the filamentous fungus Aspergillus oryzae.Scientific reports · 2018Article
- New insights from an old mutant: SPADIX4 governs fruiting body development but not hyphal fusion in Sordaria macrospora.Molecular genetics and genomics : MGG · 2017Article
- Article
- A fungal sarcolemmal membrane-associated protein (SLMAP) homolog plays a fundamental role in development and localizes to the nuclear envelope, endoplasmic reticulum, and mitochondria.Eukaryotic cell · 2015Article
- Vegetative hyphal fusion and subsequent nuclear behavior in Epichloë grass endophytes.PloS one · 2015Article
- PRO40 is a scaffold protein of the cell wall integrity pathway, linking the MAP kinase module to the upstream activator protein kinase C.PLoS genetics · 2014Article
- New insights into the roles of NADPH oxidases in sexual development and ascospore germination in Sordaria macrospora.Genetics · 2014Article
- STRIPAK complexes: structure, biological function, and involvement in human diseases.The international journal of biochemistry & cell biology · 2014Review
- The phocein homologue SmMOB3 is essential for vegetative cell fusion and sexual development in the filamentous ascomycete Sordaria macrospora.Current genetics · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted merged fluorescence (HAMF) assay for the investigation of hyphal fusion between vegetative mycelia. For this purpose, two reporter vectors were constructed encoding the histone proteins HH2B or HH2A fused at their C terminus either with the cyan or yellow fluorescent protein, respectively. The chimeric proteins generate fluorescently labeled nuclei and thus enable the distinction between different strains in a mycelial mixture. For example, hyphae with nuclei that show both cyan as well as yellow fluorescence indicate the formation of a heterokaryon as a result of hyphal fusion. To test the applicability of our HAMF assay, we used two S. macrospora developmental mutants that are supposed to have reduced hyphal fusion rates. The simple and efficient HAMF assay described here could detect even rare fusion events and should be applicable to a broad range of diverse fungal species including those lacking male or female reproductive structures or asexual spores.
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