ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2007
Real-time imaging of discrete exocytic events mediating surface delivery of AMPA receptors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 121 papers.
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Who cites it
121 citing papers in PubMed.
- Deciphering the Nanoscale Architecture of Presynaptic Actin Using a Micropatterned Presynapse-on-Glass Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- AMPA receptor diffusional trapping machinery as an early therapeutic target in neurodegenerative and neuropsychiatric disorders.Translational neurodegeneration · 2025Review
- pHusion - a robust and versatile toolset for automated detection and analysis of exocytosis.Journal of cell science · 2024Article
- Activity-dependent diffusion trapping of AMPA receptors as a key step for expression of early LTP.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Stimulus-dependent synaptic plasticity underlies neuronal circuitry refinement in the mouse primary visual cortex.Cell reports · 2024Article
- Activity-dependent mitochondrial ROS signaling regulates recruitment of glutamate receptors to synapses.eLife · 2024Article
- Synaptic memory and CaMKII.Physiological reviews · 2023Review
- Decreased Reactive Oxygen Species Signaling Alters Glutamate Receptor Transport to Synapses inmicroPublication biology · 2022Article
- The Shaping of AMPA Receptor Surface Distribution by Neuronal Activity.Frontiers in synaptic neuroscience · 2022Review
- Phosphorylation of the AMPA receptor subunit GluA1 regulates clathrin-mediated receptor internalization.Journal of cell science · 2021Article
- The biophysical basis underlying the maintenance of early phase long-term potentiation.PLoS computational biology · 2021Article
- Auxiliary Subunits Regulate the Dendritic Turnover of AMPA Receptors in Mouse Hippocampal Neurons.Frontiers in molecular neuroscience · 2021Article
- Regulation of TrkB cell surface expression-a mechanism for modulation of neuronal responsiveness to brain-derived neurotrophic factor.Cell and tissue research · 2020Review
- Reactive Oxygen Species Modulate Activity-Dependent AMPA Receptor Transport inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Imaging endocytic vesicle formation at high spatial and temporal resolutions with the pulsed-pH protocol.Nature protocols · 2020Article
- Article
- Illuminating Relationships Between the Pre- and Post-synapse.Frontiers in neural circuits · 2020Review
- Rapid actions of anti-Müllerian hormone in regulating synaptic transmission and long-term synaptic plasticity in the hippocampus.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Phosphorylation-Dependent Regulation of CaFrontiers in synaptic neuroscience · 2020Article
- Postsynaptic SNARE Proteins: Role in Synaptic Transmission and Plasticity.Neuroscience · 2019Review
61 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
We directly resolved discrete exocytic fusion events mediating insertion of AMPA-type glutamate receptors (AMPARs) to the somatodendritic surface of rat hippocampal pyramidal neurons, in slice and dissociated cultures, using protein tagging with a pH-sensitive GFP (green fluorescent protein) variant and rapid (10 frames/s) fluorescence microscopy. AMPAR-containing exocytic events occurred under basal culture conditions in both the cell body and dendrites; potentiating chemical stimuli produced an NMDA receptor-dependent increase in the frequency of individual exocytic events. The number of AMPARs inserted per exocytic event, estimated using single-molecule analysis, was quite uniform but individual events differed significantly in kinetic properties affecting the subsequent surface distribution of receptors. "Transient" events, from which AMPARs dispersed laterally immediately after surface insertion, generated a pronounced but short-lived (dissipating within approximately 1 s) increase in surface AMPAR fluorescence extending locally (2-5 microm) from the site of exocytosis. "Persistent" events, from which inserted AMPARs dispersed slowly (typically over 5-10 s), affected local surface receptor concentration to a much smaller degree. Both modes of exocytic insertion occurred throughout the dendritic shaft, but remarkably, neither mode of insertion was observed directly into synaptic spines. AMPARs entered spines preferentially from transient events occurring in the adjoining dendritic shaft, driven apparently by mass action and short-range lateral diffusion, and locally delivered AMPARs remained mostly in the mobile fraction. These results suggest a highly dynamic mechanism for both constitutive and activity-dependent surface delivery of AMPARs, mediated by kinetically distinct exocytic modes that differ in propensity to drive lateral entry of receptors to nearby synapses.
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