ArticleJournal of clinical microbiology2007
Evaluation of a multiplexed PCR assay for detection of respiratory viral pathogens in a public health laboratory setting.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Multiplex PCR system for the rapid diagnosis of respiratory virus infection: systematic review and meta-analysis.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2018Pooled it
- Accurate Detection of Avian Respiratory Viruses by Use of Multiplex PCR-Based Luminex Suspension Microarray Assay.Journal of clinical microbiology · 2016Article
- Laboratory diagnosis of viral infection.Handbook of clinical neurology · 2014Review
- Evaluation of the FilmArray® Respiratory Panel for clinical use in a large children's hospital.Journal of clinical laboratory analysis · 2013Article
- Validation of a multiplex reverse transcriptase PCR ELISA for the detection of 19 respiratory tract pathogens.Infection · 2013Article
- Detection of respiratory viruses in asymptomatic children undergoing allogeneic hematopoietic cell transplantation.Pediatric blood & cancer · 2013Article
- The microarray technology: facts and controversies.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2013Review
- Performance of the Luminex xTAG Respiratory Viral Panel Fast in a clinical laboratory setting.Journal of virological methods · 2012Article
- Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses.Journal of clinical microbiology · 2012Article
- Use and evaluation of molecular diagnostics for pneumonia etiology studies.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
- Pooling nasopharyngeal/throat swab specimens to increase testing capacity for influenza viruses by PCR.Journal of clinical microbiology · 2012Article
- Highly specific unnatural base pair systems as a third base pair for PCR amplification.Nucleic acids research · 2012Article
- Lower airway rhinovirus burden and the seasonal risk of asthma exacerbation.American journal of respiratory and critical care medicine · 2011Article
- Comparison of two multiplex methods for detection of respiratory viruses: FilmArray RP and xTAG RVP.Journal of clinical microbiology · 2011Article
- Real-world comparison of two molecular methods for detection of respiratory viruses.Virology journal · 2011Article
- Comparison of the Luminex xTAG respiratory viral panel with xTAG respiratory viral panel fast for diagnosis of respiratory virus infections.Journal of clinical microbiology · 2011Article
- Molecular diagnosis of viral respiratory infections.Current infectious disease reports · 2011Article
- Evaluation of commercial ResPlex II v2.0, MultiCode-PLx, and xTAG respiratory viral panels for the diagnosis of respiratory viral infections in adults.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- Article
- Respiratory viruses in children admitted to hospital intensive care units: evaluating the CLART® Pneumovir DNA array.Journal of medical virology · 2011Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
There are numerous viral and bacterial causes of respiratory disease. To enable rapid and sensitive detection of even the most prevalent causes, there is a need for more-simplified testing systems that enable researchers and clinicians to perform multiplexed molecular diagnostics quickly and easily. To this end, a new multiplexed molecular test called the MultiCode-PLx respiratory virus panel (PLx-RVP) was developed and then implemented in a public health laboratory setting. A total of 687 respiratory samples were analyzed for the presence of 17 viruses that commonly cause respiratory disease. As a comparator, the samples were also tested using a standard testing algorithm that included the use of a real-time influenza virus A and B reverse transcription-PCR test and routine viral culture identification. The standard testing algorithm identified 503 (73%) samples as positive and 184 as negative. Analyzing the same 687 samples, the PLx-RVP assay detected one or more targets in 528 (77%) samples and found 159 samples negative for all targets. There were 25 discordant results between the two systems; 14 samples were positive for viruses not routinely tested for by the Wisconsin State Laboratory of Hygiene, and 13 of these were confirmed by real-time PCR. When the results of the standard testing algorithm were considered "true positives," the PLx-RVP assay showed an overall sensitivity of 99% and an overall specificity of 87%. In total, the PLx-RVP assay detected an additional 40 viral infections, of which 11 were mixed infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.