ArticleJournal of clinical microbiology2007
Comparison of microsatellite length polymorphism and multilocus sequence typing for DNA-Based typing of Candida albicans.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Comparison of MultiLocus Sequence Typing (MLST) and Microsatellite Length Polymorphism (MLP) forComputational and structural biotechnology journal · 2020Article
- Genetic diversity and antifungal susceptibility of Candida albicans isolated from Iranian patients.Medical mycology · 2019Article
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- Molecular Fingerprinting Studies Do Not Support Intrahospital Transmission ofFrontiers in microbiology · 2017Article
- Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method.Iranian journal of public health · 2015Article
- Article
- Candida identification: a journey from conventional to molecular methods in medical mycology.World journal of microbiology & biotechnology · 2014Review
- Endemic genotypes of Candida albicans causing fungemia are frequent in the hospital.Journal of clinical microbiology · 2013Article
- Analysis of strain relatedness using high resolution melting in a case of recurrent candiduria.BMC microbiology · 2013Article
- Molecular fingerprints to identify Candida species.BioMed research international · 2013Review
- Candida spp. with acquired echinocandin resistance, France, 2004-2010.Emerging infectious diseases · 2012Article
- Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus.BMC microbiology · 2010Article
- Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker.Journal of clinical microbiology · 2010Article
- A new type of DNA polymorphism identified in the species-specific DNA region originating from the Candida albicans mitochondrial genome.Current microbiology · 2009Article
- Simple method to accurately differentiate Candida albicans isolates concurrently using polymorphic patterns of PCR-amplified, species-specific nuclear and mitochondrial targets.Current microbiology · 2009Article
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9 authors.
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Abstract
For genotyping Candida albicans isolates, two PCR-based methods have recently emerged: multilocus sequence typing (MLST), based on the sequence of selected genes, and microsatellite length polymorphism (MLP), based on the length of PCR products containing variable numbers of short DNA repeats. To compare the two methods in their abilities to differentiate and group C. albicans isolates, we selected 50 independent isolates collected at the National Reference Center for Mycoses and Antifungals. MLST typing was performed using sequencing of seven loci as described at (http://test1.mlst.net). The MLP method consisted of a single multiplex PCR testing three different loci. Dendrograms were constructed by the unweighted pair group cluster method with Euclidean metric for both methods. The correlation between the distance matrices was performed with a Mantel test tested with 1,000 random permutations. The sensitivity and specificity of the MLP typing system were determined after allocating MLST groups for the greater number of isolates of each distinct MLP group. The discriminatory power index was >0.99, and the distances between the isolates were highly correlated with both systems. The Mantel coefficient and the Pearson product-moment correlation coefficient were 35,699 and 0.32, respectively (P < or = 1.2 x 10(-6)). Using MLP, the average specificity and sensitivity of clustering compared to MLST were 83% and 73%, respectively, when the singletons were excluded. The two methods are similarly discriminatory and can be interchangeable depending on the objectives. MLP is less expensive and faster than MLST. However, MLST is currently more accurate and additional standardization is needed for MLP.
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