ArticleBMC infectious diseases2007
Rapid diagnosis of experimental meningitis by bacterial heat production in cerebrospinal fluid.
Article in BMC infectious diseases, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 47 citations in OpenAlex.
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- Rapid Calorimetric Detection of Bacterial Contamination: Influence of the Cultivation Technique.Frontiers in microbiology · 2019Article
- Characterization of a Listeria monocytogenes meningitis mouse model.Journal of neuroinflammation · 2018Article
- The role and reliability of rapid bedside diagnostic test in early diagnosis and treatment of bacterial meningitis.Indian journal of pediatrics · 2015Article
- Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label-free well-plate assay.Biotechnology journal · 2015Article
- Epidemiology and Risk Factors Associated with Developing Bacterial Meningitis among Children in Gaza Strip.Iranian journal of public health · 2014Article
- Propionibacterium acnes: from commensal to opportunistic biofilm-associated implant pathogen.Clinical microbiology reviews · 2014Review
- Activities of fosfomycin and rifampin on planktonic and adherent Enterococcus faecalis strains in an experimental foreign-body infection model.Antimicrobial agents and chemotherapy · 2014Article
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- Comparative analysis of Staphylococcus aureus and Escherichia coli microcalorimetric growth.BMC microbiology · 2013Article
- Periprosthetic joint infections: clinical and bench research.TheScientificWorldJournal · 2013Review
- Isothermal microcalorimetry, a new tool to monitor drug action against Trypanosoma brucei and Plasmodium falciparum.PLoS neglected tropical diseases · 2012Article
- Gentamicin improves the activities of daptomycin and vancomycin against Enterococcus faecalis in vitro and in an experimental foreign-body infection model.Antimicrobial agents and chemotherapy · 2011Article
- Oxidative stress, cytokine/chemokine and disruption of blood-brain barrier in neonate rats after meningitis by Streptococcus agalactiae.Neurochemical research · 2011Article
- MicroDSC study of Staphylococcus epidermidis growth.BMC microbiology · 2010Article
- Chip calorimetry for fast and reliable evaluation of bactericidal and bacteriostatic treatments of biofilms.Antimicrobial agents and chemotherapy · 2010Article
- The meningococcal ABC-Type L-glutamate transporter GltT is necessary for the development of experimental meningitis in mice.Infection and immunity · 2009Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCalorimetry is a nonspecific technique which allows direct measurement of heat generated by biological processes in the living cell. We evaluated the potential of calorimetry for rapid detection of bacterial growth in cerebrospinal fluid (CSF) in a rat model of bacterial meningitis.
methodsInfant rats were infected on postnatal day 11 by direct intracisternal injection with either Streptococcus pneumoniae, Neisseria meningitidis or Listeria monocytogenes. Control animals were injected with sterile saline or heat-inactivated S. pneumoniae. CSF was obtained at 18 hours after infection for quantitative cultures and heat flow measurement. For calorimetry, 10 microl and 1 microl CSF were inoculated in calorimetry ampoules containing 3 ml trypticase soy broth (TSB).
resultsThe mean bacterial titer (+/- SD) in CSF was 1.5 +/- 0.6 x 108 for S. pneumoniae, 1.3 +/- 0.3 x 106 for N. meningitidis and 3.5 +/- 2.2 x 104 for L. monocytogenes. Calorimetric detection time was defined as the time until heat flow signal exceeded 10 microW. Heat signal was detected in 10-microl CSF samples from all infected animals with a mean (+/- SD) detection time of 1.5 +/- 0.2 hours for S. pneumoniae, 3.9 +/- 0.7 hours for N. meningitidis and 9.1 +/- 0.5 hours for L. monocytogenes. CSF samples from non-infected animals generated no increasing heat flow (<10 microW). The total heat was the highest in S. pneumoniae ranging from 6.7 to 7.5 Joules, followed by L. monocytogenes (5.6 to 6.1 Joules) and N. meningitidis (3.5 to 4.4 Joules). The lowest detectable bacterial titer by calorimetry was 2 cfu for S. pneumoniae, 4 cfu for N. meningitidis and 7 cfu for L. monocytogenes.
conclusionBy means of calorimetry, detection times of <4 hours for S. pneumoniae and N. meningitidis and <10 hours for Listeria monocytogenes using as little as 10 microl CSF were achieved. Calorimetry is a new diagnostic method allowing rapid and accurate diagnosis of bacterial meningitis from a small volume of CSF.
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